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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • RF1ML Antibodies
          The product (cat # {{productObj.productId}}) that you're looking for has been discontinued and replaced with {{productObj.replacementProductId}}. The product (cat # {{productObj.productId}}) that you're looking for has been discontinued. Please contact Technical Support with any questions.

          Invitrogen

          RF1ML Polyclonal Antibody

          View all (16) RF1ML antibodies

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          Cite RF1ML Polyclonal Antibody

          • Antibody Testing Data (3)
          RF1ML Antibody in Immunocytochemistry (ICC/IF)
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          RF1ML Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          RF1ML Antibody (PA5-112181) in ICC/IF

          Immunocytochemistry-Immunofluorescence analysis of RF1ML in HT29 cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with RF1ML Polyclonal Antibody (Product # PA5-112181) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Nucleus and Cytoplasm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          RF1ML Antibody in Immunocytochemistry (ICC/IF)
          RF1ML Antibody in Western Blot (WB)
          RF1ML Antibody in Immunoprecipitation (IP)

          Product Details

          PA5-112181

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -

          Immunoprecipitation (IP)

          5-10 µL/mg lysate
          -
          Product Specifications

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E. coli-derived Human MTRF1L fragment

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2866917

          Target Information

          Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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