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|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to human Replication factor C subunit 3.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||5 µg/ml|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-27673 detects RFC3 from human and rat samples.
PA1-27673 may be successfully used in ELISA and Western blot applications. By Western blot, this antibody has only been tested on fusion protein.
The immunogen is a synthetic peptide corresponding to human Replication factor C subunit 3.
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. The elongation of primed DNA templates by DNA polymerase delta and DNA poly
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Replication factor C3 is a CREB target gene that regulates cell cycle progression through the modulation of chromatin loading of PCNA.
PA1-27673 was used in western blot to study CREB in acute myeloid leukemia
|Chae HD,Mitton B,Lacayo NJ,Sakamoto KM||Leukemia (29:1379)||2015|
A1 38 kDa subunit; activator 1 38 kDa subunit; activator 1 subunit 3; MGC5276; replication factor C 3; replication factor C 38 kDa subunit; replication factor C subunit 3; RF-C 38 kDa subunit; RFC, 38 kD subunit; RFC38