|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide corresponding to a portion of human Replication Factor C 437 kDa subunit encoded in part by exons 10 and 11.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris phosphate/tris citrate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26570 detects RFC4 from human samples. PA1-26570 is expected to cross react with mouse due to sequence homology.
PA1-26570 has been successfully used in Western blot procedures.
The PA1-26570 immunogen is a synthetic peptide corresponding to a portion of human Replication Factor C 437 kDa subunit encoded in part by exons 10 and 11.
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.