Invitrogen

RFC4 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (16) RFC4 antibodies

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Datasheet

Protocols

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RFC4 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of RFC4 in human testis and kidney tissues using a RFC4 polyclonal antibody (Product # PA5-84382). Corresponding RNA-seq data are presented for the same tissues.

RFC4 Antibody in Western Blot (WB) — Western blot analysis of RFC4 by a RFC4 polyclonal antibody (Product # PA5-84382). Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG.

RFC4 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of RFC4 demonstrates antibody specificity. Immunohistochemical analysis of RFC4 using anti-RFC4 Polyclonal Antibody (Product #PA5-84382), shows significant staining of RFC4 in testis and shows minimal or weak staining in kidney tissues. The relative expression levels of RFC4 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-84382

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human RFC4 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.05 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2791534

Product Specific Information

Immunogen sequence: VVVENNLSDK QKSIITEKLA EVDKCLADGA DEHLQLISLC ATVMQQLSQN

Target Information

Human Replication Factor C (RFC) is a hetero-pentameric protein consisting of the p140, p40, p38, p37 and p36 subunits. RFC functions in the loading of the DNA polymerase delta processivity factor (PCNA, Proliferating Cell nuclear Antigen) onto a primed DNA template during DNA synthesis and repair. RFC may also participate in the DNA damage checkpoint response with a PCNA-like hetero-trimer consisting of Hus 1, Rad 1 and Rad 9.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A1 37 kDa subunit; Activator 1 37 kDa subunit; Activator 1 subunit 4; replication factor C (activator 1) 4, 37kDa; Replication factor C 37 kDa subunit; Replication factor C subunit 4; RF-C 37 kDa subunit; RFC 37 kDa subunit; RFC p37

Gene Aliases: A1; RFC37; RFC4

UniProt ID: (Human) P35249

Entrez Gene ID: (Human) 5984

Molecular Function: DNA-directed DNA polymerase