Invitrogen

RFC4 Polyclonal Antibody

Product Image Gallery

RFC4 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of RFC4 in human testis and kidney tissues using a RFC4 polyclonal antibody (Product # PA5-84382). Corresponding RNA-seq data are presented for the same tissues.

RFC4 Antibody in Western Blot (WB) — Western blot analysis of RFC4 by a RFC4 polyclonal antibody (Product # PA5-84382). Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RFC4

Conjugate

Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Immunogen sequence: VVVENNLSDK QKSIITEKLA EVDKCLADGA DEHLQLISLC ATVMQQLSQN

Target Information

Human Replication Factor C (RFC) is a hetero-pentameric protein consisting of the p140, p40, p38, p37 and p36 subunits. RFC functions in the loading of the DNA polymerase delta processivity factor (PCNA, Proliferating Cell nuclear Antigen) onto a primed DNA template during DNA synthesis and repair. RFC may also participate in the DNA damage checkpoint response with a PCNA-like hetero-trimer consisting of Hus 1, Rad 1 and Rad 9.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: A1 37 kDa subunit; Activator 1 37 kDa subunit; Activator 1 subunit 4; replication factor C (activator 1) 4, 37kDa; Replication factor C 37 kDa subunit; Replication factor C subunit 4; RF-C 37 kDa subunit; RFC 37 kDa subunit; RFC37

Gene Aliases: A1; RFC37; RFC4

UniProt ID: (Human) P35249

Entrez Gene ID: (Human) 5984

Molecular Function: DNA binding protein DNA-directed DNA polymerase nucleic acid binding nucleotidyltransferase transferase