Invitrogen

RFP2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (4) RFP2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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RFP2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RFP2 in human testis and duodenum tissues using RFP2 Polyclonal Antibody (Product # PA5-51377). Corresponding TRIM13 RNA-seq data are presented for the same tissues.

RFP2 Antibody in Western Blot (WB) — Western blot analysis of RFP2 in control (vector only transfected HEK293T lysate) and TRIM13 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RFP2 Polyclonal Antibody (Product # PA5-51377).

RFP2 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of RFP2 demonstrates antibody specificity. Immunohistochemical analysis of RFP2 using anti-RFP2 Polyclonal Antibody (Product # PA5-51377), shows significant staining of RFP2 in human testis and shows minimal or weak staining in human duodenum tissues. The relative expression levels of RFP2 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-51377

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human RFP2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2646489

Product Specific Information

Immunogen sequence: LSRLDTLETS KRKSLQLLTK DSDKVKEFFE KLQHTLDQKK NEILSDFETM KLAVMQAYDP EINKLNTILQ EQRMAFNIAE AFKDVSEPIV FLQQMQEFRE KIKVIKETPL PPSNLPASPL MKNFDTSQWE DIKLVDVDKL SLP

Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 97%.

Target Information

The protein encoded by the RFP2 gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies near the nucleus, but its function is unknown. The gene is located on chromosome 13 within the minimal deletion region for B-cell chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-cell chronic lymphocytic leukemia tumor suppressor Leu5; CLL-associated RING finger; E3 ubiquitin-protein ligase TRIM13; Leukemia-associated protein 5; Putative tumor suppressor RFP2; Ret finger protein 2; RING finger protein 77; RING-type E3 ubiquitin transferase TRIM13; tripartite motif protein 13; tripartite motif-containing 13; Tripartite motif-containing protein 13

Gene Aliases: CAR; DLEU5; LEU5; RFP2; RNF77; TRIM13

UniProt ID: (Human) O60858

Entrez Gene ID: (Human) 10206

Molecular Function: scaffold/adaptor protein ubiquitin-protein ligase