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Bethyl Laboratories
RFX1 Polyclonal Antibody
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FIGURE: 1 / 1
RFX1 Antibody (A303-042A) in IP
Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RFX1 antibody (Product # A303-042A) used for IP at 6 µg/mg lysate. RFX1 was also immunoprecipitated by rabbit anti-RFX1 antibodies BL11050 and A303-043A, which recognize other epitopes. For blotting immunoprecipitated RFX1, BL11050 was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A303-042A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 200 and 250 of Human Regulatory Factor X, 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EF-C; Enhancer factor C; MHC class II regulatory factor RFX; MHC class II regulatory factor RFX1; Regulatory factor X 1; regulatory factor X, 1 (influences HLA class II expression); RFX; RFX gene; trans-acting regulatory factor 1; Transcription factor RFX1
Gene Aliases: EFC; RFX; RFX1
UniProt ID: (Human) P22670
Entrez Gene ID: (Human) 5989
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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