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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide of human Regulatory Factor X 5 region encoded within exon 5 (LocusLinkID 5993)|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris phosphate/tris citrate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Immunoprecipitation (IP)||1-4 µl|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-22854 detects RFX5 in Human samples.
PA1-22854 has been successfully used in Immunoprecipitation and Western Blot procedures.
PA1-22854 immunogen corresponds to Synthetic peptide of human Regulatory Factor X 5 region encoded within exon 5 (LocusLinkID 5993).
A lack of MHC class II expression results in a severe immunodeficiency syndrome called MHC class II deficiency, or the bare lymphocyte syndrome type II (BLS). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC class II promoters. The lack of RFX binding activity in complementation group C results from mutations in the gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). This gene was called RFX5 because it is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DNA-binding protein RFX5; Regulatory factor X 5; regulatory factor X, 5 (influences HLA class II expression); RP11-126K1.5