Western blot detection of RFX5 in 35µg per lane of (lane 1) Raji B cell nuclear extract lysates compared to a control with no antibody (lane 2) using a RFX5 polyclonal antibody (Product # PA1-32133) at a dilution of 1:2,500 for overnight at 4°C. Secondary detection was performed using a HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at room temperature. Samples were blocked 5% BLOTTO overnight at 4°C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH, corresponding to amino acids 320-494 of Human RFX 5.|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||0.5-1 µl|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32133 detects RFX5 from mouse, human samples.
PA1-32133 has been successfully used in immunoprecipitation, Western blot applications.
The PA1-32133 immunogen is: Synthetic peptide conjugated to KLH, corresponding to amino acids 320-494 of Human RFX 5.
A lack of MHC class II expression results in a severe immunodeficiency syndrome called MHC class II deficiency, or the bare lymphocyte syndrome type II (BLS). At least 4 complementation groups have been identified in B-cell lines established from patients
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