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Agrisera
RGA/DELLA protein RGA Polyclonal Antibody
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FIGURE: 1 / 2
RGA/DELLA protein RGA Antibody (AS11-1630) in WB
Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µL of 2.5 x LaemLi for 80-120 mg of homogenized material) and boiled in 2,5 x LaemmLi Buffer (WITH 60 Mm DTT final concentration) otherwise RGA protein will degrade. Plants were grown on 1/ MS for 15 days and were treated with 1 µM GA for 2 hours (GA+) or without hormone (GA-). Total protein extracts were denatured for 2 min. at 95°C and wer... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
AS11-1630
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide chosen from RGA of Arabidopsis thaliana.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
RGA, also called repressor of ga1 3 like, is a DELLA protein that represses gibberellin signaling in plants. In the absence of gibberellin, RGA accumulates in the nucleus and binds transcription factors to inhibit growth processes such as stem elongation, seed germination, and flowering. When gibberellin levels rise, the receptor GID1 promotes interaction of RGA with the SCF E3 ligase containing SLY1 or GID2, leading to ubiquitination and proteasomal degradation of RGA and activation of GA responsive genes. RGA integrates cross talk with auxin, brassinosteroids, and light signaling, and its stability and activity are modulated by phosphorylation and other posttranslational modifications. Mutations affecting RGA alter plant stature, developmental timing, and stress responses by shifting the balance of growth repression and hormone sensitivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT2G01570; F2I9.19; F2I9_19; Q9SLH3; REPRESSOR OF GA; REPRESSOR OF GA1-3 1; RGA; RGA1
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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