Search
Invitrogen
RGMA Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
RGMA Antibody (PA5-47326) in IHC (P)
Immunohistochemical analysis of RGMA in immersion fixed paraffin-embedded sections of human brain (spinal cord). Samples were incubated in RGMA polyclonal antibody (Product # PA5-47326) using a dilution of 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using the Anti-Go... View More
Product Details
PA5-47326
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RGM-A Cys48-Gly422
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, less than 40% cross-reactivity with recombinant chicken RGM and recombinant mouse RGM-A is observed, and less than 1% cross-reactivity with recombinant human (rh) RGM-B and rhRGM-C is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Repulsive guidance molecule A; repulsive guidance molecule family member a; RGM domain family member A; RGM domain family, member A; unnamed protein product
Gene Aliases: RGM; RGMA
UniProt ID: (Human) Q96B86
Entrez Gene ID: (Human) 56963
neural tube closure
membrane protein ectodomain proteolysis
positive regulation of macromolecule metabolic process
positive regulation of neuron projection development
negative regulation of neuron projection development
BMP signaling pathway
regulation of BMP signaling pathway
neuron projection development
positive regulation of transcription from RNA polymerase II promoter
negative regulation of collateral sprouting
negative regulation of axon regeneration
positive regulation of membrane protein ectodomain proteolysis
regulation of primary metabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support