|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 209 of Human RGS1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-29579 targets RGS1 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-29579 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 209 of Human RGS1.
This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effect of interferon-ß1b on CXCR4-dependent chemotaxis in T cells from multiple sclerosis patients.
PA5-29579 was used in flow cytometry to elucidate the mechanism by which IFN-beta1b suppresses relapsing-remitting multiple sclerosis
|Wostradowski T,Gudi V,Pul R,Gingele S,Lindquist JA,Stangel M,Lindquist S||Clinical and experimental immunology (182:162)||2015|