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Invitrogen
RGS14 Polyclonal Antibody
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FIGURE: 1 / 5
RGS14 Antibody (PA5-97309) in IHC (P)
Immunohistochemistry analysis of RGS14 in paraffin-embedded human thyroid cancer. Samples were incubated with RGS14 Polyclonal antibody (Product # PA5-97309) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-97309
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 230-510 of human RGS14 (NP_0064712)
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PPVEGPGGRP LRKSFRRELG GTANAALRRE SQGSLNSSAS LDLGFLAFVS SKSESHRKSL GSTEGESESR PGKYCCVYLP DGTASLALAR PGLTIRDMLA GICEKRGLSL PDIKVYLVGN EQALVLDQDC TVLADQEVRL ENRITFELEL TALERVVRIS AKPTKRLQEA LQPILEKHGL SPLEVVLHRP GEKQPLDLGK LVSSVAAQRL VLDTLPGVKI SKARDKSPCR SQGCPPRTQD KATHPPPASP SSLVKVPSSA TGKRQTCDIE GLVELLNRVQ S; Positive Samples: SH-SY5Y, Mouse kidney; Cellular Location: Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus, PML body, centrosome, cytoskeleton, dendrite, dendritic spine, microtubule organizing center, postsynaptic cell membrane, postsynaptic density, spindle, spindle pole, synapse
Target Information
RGS14 is a member of the regulator of G-protein signaling family which contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. RGS14 attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. The resulting hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of RGS14 have been observed but have not been thoroughly characterized. Further, RGS14 acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism; plays a role in cell division; is required for the nerve growth factor (NGF)-mediated neurite outgrowth, and is involved in stress resistance. RGS14 protein may also be involved in visual memory processing capacity and hippocampal-based learning and memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: rap1/rap2 interacting protein; RAP1/RAP2-interacting protein; Regulator of G-protein signaling 14; regulator of G-protein signalling 14; RGS14; RGSE; RPIP1
Gene Aliases: RGS14; RPIP1
UniProt ID: (Human) O43566, (Mouse) P97492, (Rat) O08773
Entrez Gene ID: (Human) 10636, (Mouse) 51791, (Rat) 114705
nucleocytoplasmic transport
response to oxidative stress
cell cycle
spindle organization
chromosome segregation
mitotic nuclear division
signal transduction
G-protein coupled receptor signaling pathway
multicellular organism development
learning
long-term memory
regulation of G-protein coupled receptor protein signaling pathway
visual learning
negative regulation of signal transduction
zygote asymmetric cell division
negative regulation of synaptic plasticity
intracellular signal transduction
termination of G-protein coupled receptor signaling pathway
negative regulation of MAP kinase activity
positive regulation of GTPase activity
regulation of DNA-templated transcription in response to stress
platelet-derived growth factor receptor signaling pathway
positive regulation of neurogenesis
cell division
long-term synaptic potentiation
negative regulation of ERK1 and ERK2 cascade
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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