Western blot analysis of RGS2 in (lane 2) Gene specific transient over-expression HEK293T cell lysate and (lane 3) Empty vector transfected control HEK293T cell lysate. Samples were probed with a RGS2 polyclonal antibody (Product # PA1-29877) at a dilution of 1:500. Secondary detection was performed using a peroxidase conjugated Rabbit anti-Chicken IgY (IgG) at a 1:10,000 dilution and developed with TMB Substrate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Full length human RGS2 (Amino acids 1-211)|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29877 detects RGS2 from rat, human, mouse samples.
PA1-29877 has been successfully used in Western blot applications.
The PA1-29877 immunogen is: Full length human RGS2 (Amino acids 1-211)
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.