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Invitrogen
RHD Polyclonal Antibody
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FIGURE: 1 / 1
RHD Antibody (PA5-90215) in WB
Western blot analysis of RHD in extracts of various cell lines. Samples were incubated with RHD Polyclonal antibody (Product # PA5-90215) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-90215
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human RHD (NP_0011211631)
Conjugate
Form
Liquid
Concentration
2.4 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LGNLRMVISN IFNTDYHMNM MHIYVFAAYF GLSVAWCLPK PLPEGTEDKD QTATIPSLSA MLGALFLWMF WPSFNSALLR SPIERKNAVF NTYYAVAVSV V; Positive Samples: U-251MG, LO2, rat spleen, rat liver; Cellular Location: Membrane, Multi-pass membrane protein
Target Information
The Rhesus (Rh) blood group system represents one of the most complex and important systems in humans. Two highly homologous genes RHD and RHCE (collectively referred to as RH30 or RHCED) encode the antigens of the Rh blood group system (1-4). These tightly linked genes map to human chromosomal position 1p34.1-1p36. The RHD gene, which is commonly deleted from a large segment of the population, encodes the most potent blood group immunogen, the D antigen (2,5). Rh incompatibility between maternal and fetal blood types results in hemolytic disease of the newborn (HDN), which often results in fetal death (1,2,6). The RHCE gene exists in four allelic forms, and each allele determines the expression of two antigens in Ce, ce, cE, or CE combinations (2,4,5). The RHCED antigens exist as integral membrane proteins with contain 12-transmembrane helices, and maintain erythrocyte membrane integrity. The presentation of the Rh antigenic activity requires the formation of a complex between the RHCED antigens and RHAG (RH50).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: blood group antigen D; blood group protein RHD; Blood group Rh(D) polypeptide; CD240D; D antigen (DCS); Erythrocyte membrane glycoprotein Rh30; Rh blood group antigen Evans; Rh blood group, D anitgen; Rh blood group, D antigen; RH polypeptide 2; RhD blood group antigen; RhD polypeptide; Rhesus blood group CE and D; Rhesus blood group D antigen allele DIII type 7; Rhesus D antigen; Rhesus system D polypeptide; RhPII; RHXIII; truncated rhesus blood group D antigen; truncated rhesus D
Gene Aliases: CD240D; DIIIc; RH; RH30; Rh4; RHCED; RHD; RhDCw; RHDel; RHDVA(TT); RhII; RhK562-II; RhPI; RHPII; RHXIII
UniProt ID: (Human) Q02161, (Rat) O88298
Entrez Gene ID: (Human) 6007, (Rat) 60414
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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