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Invitrogen

RIP1 Polyclonal Antibody

View all (44) RIP1 antibodies

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Product Details

PA5-34778

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIP. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2552130

Product Specific Information

Recommended positive controls: HUVEC.

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell death protein RIP; FLJ39204; OTTHUMP00000015955; receptor (TNFRSF)-interacting serine-threonine kinase 1; Receptor-interacting protein 1; receptor-interacting protein kinase 1; Receptor-interacting serine/threonine-protein kinase 1; RIP-1; RIP1 (CT); serine/threonine-protein kinase RIP; unnamed protein product

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Gene Aliases: AIEFL; IMD57; RIP; RIP-1; RIP1; RIPK1

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UniProt ID: (Human) Q13546

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Entrez Gene ID: (Human) 8737

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity JUN kinase kinase kinase activity death receptor binding protein binding ATP binding kinase activity transferase activity ubiquitin protein ligase binding signaling adaptor activity identical protein binding protein homodimerization activity macromolecular complex binding death domain binding protein serine kinase activity
Process(es)
MAPK cascade positive regulation of protein phosphorylation apoptotic process inflammatory response response to oxidative stress signal transduction I-kappaB kinase/NF-kappaB signaling positive regulation of gene expression programmed cell death protein catabolic process positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production tumor necrosis factor-mediated signaling pathway response to tumor necrosis factor intracellular signal transduction peptidyl-serine autophosphorylation positive regulation of phosphorylation positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of programmed cell death positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade positive regulation of neuron apoptotic process positive regulation of macrophage differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade protein autophosphorylation positive regulation of inflammatory response positive regulation of NF-kappaB transcription factor activity regulation of necroptotic process positive regulation of necroptotic process negative regulation of necroptotic process positive regulation of programmed necrotic cell death positive regulation of interleukin-6-mediated signaling pathway T cell apoptotic process necroptotic process cellular response to hydrogen peroxide cellular response to tumor necrosis factor cellular response to growth factor stimulus extrinsic apoptotic signaling pathway programmed necrotic cell death ripoptosome assembly necroptotic signaling pathway positive regulation of execution phase of apoptosis ripoptosome assembly involved in necroptotic process positive regulation of NIK/NF-kappaB signaling positive regulation of tumor necrosis factor-mediated signaling pathway positive regulation of production of miRNAs involved in gene silencing by miRNA amyloid fibril formation regulation of reactive oxygen species metabolic process positive regulation of reactive oxygen species metabolic process negative regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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