|Tested species reactivity||Human|
|Published species reactivity||Rat, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues G(718) N P T K G R V K K K E K T P L R D(735) of human RIP140.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-841 detects recombinant human Receptor-Interacting Protein 140 (RIP 140).
PA1-841 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects two proteins at ~140 kDa representing human RIP 140 expressed in rabbit reticulocyte lysate.
The PA1-841 immunogen is a synthetic peptide corresponding to residues G(718) N P T K G R V K K K E K T P L R D(735) of human RIP140. This immunizing peptide (Cat. # PEP-030) is available for use in neutralization and control experiments.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate.
Receptor-Interacting Protein 140 (RIP140) has been shown to enhance estrogen receptor transcriptional activity through the AF2 activation domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPAR alpha) and has been proposed to competitively inhibit PPAR alpha interaction with Steroid Receptor Coactivator-1 (SRC-1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The role of receptor-interacting protein 140 in the accumulation of fat in ovariectomised rats.
PA1-841 was used in western blot to investigate the role of receptor-interacting protein 140 in obesity progression induced by ovariectomy
|Liu WH,Lee YM,Lam KK,Chen YF,Wang JJ,Yen MH,Cheng PY||Obesity surgery (21:935)||2011|
|Non-human primate||Not Cited||
Nuclear factor RIP140 modulates transcriptional activation by the estrogen receptor.
PA1-841 was used in immunocytochemistry to investigate the effect of RIP140 on estrogen-mediated transcriptional activation
|Cavaillès V,Dauvois S,L'Horset F,Lopez G,Hoare S,Kushner PJ,Parker MG||The EMBO journal (14:3741)||1995|