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Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.1-0.5 µg/mL | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
5 µg/mL | - |
Immunoprecipitation (IP) |
20 µg/mL | - |
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Mouse, Rat, Human |
Published species |
Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A 14 amino acid peptide near the carboxy terminus of murine RIP3 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
0.02% sodium azide |
Storage conditions |
4° C |
RRID |
AB_11154832 |
A suggested positive control is NIH-3T3 cell lysate.
PA5-19956 can be used with blocking peptide PEP-0081.
The PA5-19956 immunogen is located within the last 50 amino acids of RIP3.
Predicted molecular ~ 53kD for mouse RIP3 and 57kD for human RIP3. In Western blot applications, this antibody has been observed to detect a band at: 53kD for mouse RIP3 and 57kD for human RIP3
RIP3 is a novel member of RIP Ser/Thr kinase family. It is a potent inducer of apoptosis and is an important component of TNFR1 signaling complex. RIP3 consists of an N-terminal RIP-like-Kinase domain and a unique, non-homologous C-terminus responsible for caspase activation and apoptosis induction. RIP3 is expressed in multiple tissues including hematopoietic cells and plays a functional role in the regulation of apoptosis and NF-kB signaling. It is recruited to the TNFR1 signaling complex in a RIP-dependant manner where it induces apoptosis by activating caspases and/or inhibiting TNFR1-induced NF-kB activation. RIP3 negatively regulates the TLR3-Trif-mediated NF-kB signaling pathway by competing with the binding of Trif to RIP1. The human RIP3 gene is localized in the chromosomal region 14q11.2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Homocysteine respondent protein HCYP2; mRIP3; receptor interacting protein 3; Receptor-interacting protein 3; receptor-interacting serine-threonine kinase 3; Receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3
Gene Aliases: 2610528K09Rik; AW107945; Hcyp2; RIP3; RIPK3
UniProt ID: (Human) Q9Y572, (Mouse) Q9QZL0, (Rat) Q9Z2P5
Entrez Gene ID: (Human) 11035, (Mouse) 56532, (Rat) 246240
Molecular Function:
non-receptor serine/threonine protein kinase
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