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Invitrogen
RIP3 Polyclonal Antibody
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FIGURE: 1 / 7
RIP3 Antibody (PA5-19956) in WB
Western Blot Validation in C2C12 Cells Mouse. Loading: 15 µg of lysates per lane. Antibodies: RIP3 Polyclonal Antibody (Product # PA5-19956) 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: RIP3 Polyclonal Antibody, 0.1 µg/mL in the presence of peptide blocking Lane 2: RIP3 Polyclonal Antibody, 0.1 µg/mL Lane 3: RIP3 Polyclonal Antibody, 0.2 µg/mL Lane 4: RIP3 Polyclonal Antibody, 0.5 µg/mL
Product Details
PA5-19956
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to 14 amino acids near the carboxy terminus of murine RIP3. The immunogen is located within the last 50 amino acids of RIP3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive controls are Rat Thymus Lysate, 3T3/NIH Cell Lysate, or C2C12 Cell Lysate.
PA5-19956 can be used with blocking peptide PEP-0081.
Target Information
RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, which contains a C-terminal domain unique from other RIP family members. RIPK3 is predominantly localized to the cytoplasm, and specifically, are colocalized in the mitochondrion which can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. RIPK3 is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor and caspase-8 prevents RIPK3-dependent necrosis without inducing apoptosis by functioning in a proteolytically active complex with CFLAR and that this complex is required for the protective function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Homocysteine respondent protein HCYP2; mRIP3; receptor interacting protein 3; Receptor-interacting protein 3; Receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3
Gene Aliases: 2610528K09Rik; AW107945; Hcyp2; Rip3; Ripk3
UniProt ID: (Mouse) Q9QZL0, (Rat) Q9Z2P5
Entrez Gene ID: (Mouse) 56532, (Rat) 246240
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
NF-kappaB-inducing kinase activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein complex binding
identical protein binding
non-receptor serine/threonine protein kinase
regulation of T cell mediated cytotoxicity
regulation of adaptive immune response
protein phosphorylation
I-kappaB kinase/NF-kappaB signaling
positive regulation of phosphatase activity
programmed cell death
viral process
phosphorylation
signal transduction by protein phosphorylation
activation of protein kinase activity
regulation of interferon-gamma production
T cell differentiation in thymus
NIK/NF-kappaB signaling
T cell homeostasis
regulation of activated T cell proliferation
protein autophosphorylation
lymph node development
spleen development
thymus development
positive regulation of NF-kappaB transcription factor activity
protein homooligomerization
protein heterooligomerization
positive regulation of transferase activity
positive regulation of ligase activity
positive regulation of oxidoreductase activity
positive regulation of necroptotic process
regulation of activation-induced cell death of T cells
necroptotic process
positive regulation of protein deacetylation
amyloid fibril formation
regulation of reactive oxygen species metabolic process
positive regulation of reactive oxygen species metabolic process
regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation
positive regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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