A suggested positive control is NIH-3T3 cell lysate.
PA5-19956 can be used with blocking peptide PEP-0081.
The PA5-19956 immunogen is located within the last 50 amino acids of RIP3.
Predicted molecular ~ 53kD for mouse RIP3 and 57kD for human RIP3. In Western blot applications, this antibody has been observed to detect a band at: 53kD for mouse RIP3 and 57kD for human RIP3
Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kappa-B activation. Overexpression of RIP3 induces apoptosis and NF-kappa-B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.
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Protein Aliases: Homocysteine respondent protein HCYP2; mRIP3; receptor interacting protein 3; Receptor-interacting protein 3; receptor-interacting serine-threonine kinase 3; Receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3
Gene Aliases: 2610528K09Rik; AW107945; Hcyp2; RIP3; RIPK3