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Product Details
H00008767-D01P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
RIPK2 (NP_003812.1, 1 a.a. approximately 540 a.a) full-length human protein.
Conjugate
Unconjugated
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
Sequence of this protein is as follows: MNGEAICSAL PTIPYHKLAD LRYLSRGASG TVSSARHADW RVQVAVKHLH IHTPLLDSER KDVLREAEIL HKARFSYILP ILGICNEPEF LGIVTEYMPN GSLNELLHRK TEYPDVAWPL RFRILHEIAL GVNYLHNMTP PLLHHDLKTQ NILLDNEFHV KIADFGLSKW RMMSLSQSRS SKSAPEGGTI IYMPPENYEP GQKSRASIKH DIYSYAVITW EVLSRKQPFE DVTNPLQIMY SVSQGHRPVI NEESLPYDIP HRARMISLIE SGWAQNPDER PSFLKCLIEL EPVLRTFEEI TFLEAVIQLK KTKLQSVSSA IHLCDKKKME LSLNIPVNHG PQEESCGSSQ LHENSGSPET SRSLPAPQDN DFLSRKAQDC YFMKLHHCPG NHSWDSTISG SQRAAFCDHK TTPCSSAIIN PLSTAGNSER LQPGIAQQWI QSKREDIVNQ MTEACLNQSL DALLSRDLIM KEDYELVSTK PTRTSKVRQL LDTTDIQGEE FAKVIVQKLK DNKQMGLQPY PEILVVSRSP SLNLLQNKSM
Target Information
RIPK2 is a serine/threonine kinase that is involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CARD-carrying kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; EC 2.7.11.1; growth-in; growth-inhibiting gene 30; kinase RIPK2; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase; Receptor-interacting protein 2; receptor-interacting serine-threonine kinase 2; Receptor-interacting serine/threonine-protein kinase 2; RIP 2; RIP-2; RIP-like; RIP-like-interacting CLARP kinase; Tyrosine-protein kinase RIPK2; WUGSC:H_RG437L15.1
Gene Aliases: CARD3; CARDIAK; CCK; GIG30; RICK; RIP2; RIPK2; UNQ277/PRO314/PRO34092
UniProt ID: (Human) O43353
Entrez Gene ID: (Human) 8767
Molecular Function:
non-receptor serine/threonine protein kinase
activation of MAPK activity
positive regulation of cytokine-mediated signaling pathway
adaptive immune response
apoptotic process
inflammatory response
signal transduction
I-kappaB kinase/NF-kappaB signaling
JNK cascade
positive regulation of peptidyl-threonine phosphorylation
peptidyl-tyrosine phosphorylation
positive regulation of protein ubiquitination
lipopolysaccharide-mediated signaling pathway
positive regulation of chemokine production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
positive regulation of interleukin-2 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of immature T cell proliferation
positive regulation of peptidyl-serine phosphorylation
toll-like receptor 2 signaling pathway
toll-like receptor 4 signaling pathway
T cell proliferation
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
innate immune response
positive regulation of T-helper 1 cell differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
positive regulation of alpha-beta T cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-positive bacterium
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
positive regulation of ERK1 and ERK2 cascade
nucleotide-binding oligomerization domain containing signaling pathway
nucleotide-binding oligomerization domain containing 1 signaling pathway
nucleotide-binding oligomerization domain containing 2 signaling pathway
response to interleukin-1
response to interleukin-12
response to interleukin-18
cellular response to lipoteichoic acid
cellular response to peptidoglycan
cellular response to muramyl dipeptide
positive regulation of xenophagy
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