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FIGURE: 1 / 1
RIPX Antibody (PA5-88846) in WB
Western blot analysis of RIPX in extracts of various cell lines. Samples were incubated with RIPX Polyclonal antibody (Product # PA5-88846) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88846
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human RUFY3 (NP_0557761)
Conjugate
Form
Liquid
Concentration
0.46 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805168
Product Specific Information
Immunogen sequence: MSALTPPTDM PTPTTDKITQ AAMETIYLCK FRVSMDGEWL CLRELDDISL TPDPEPTHED PNYLM; Positive Samples: Mouse brain, Rat brain; Cellular Location: Cell projection, Cytoplasm, Endomembrane system, Perikaryon, filopodium, growth cone, invadopodium, lamellipodium
Target Information
Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein RUFY3; rap2 interacting protein x; Rap2-interacting protein x; RIPx; RUN and FYVE domain-containing protein 3; Singar; Singar1; singar2; Single axon-regulated protein; Single axon-regulated protein 1
Gene Aliases: 2810428M05Rik; 6330416M07Rik; AW455998; AW538594; D5Bwg0860e; Ripx; Rpipx; Rufy3; Singar1
UniProt ID: (Mouse) Q9D394, (Rat) Q5FVJ0
Entrez Gene ID: (Mouse) 52822, (Rat) 360921
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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