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Bethyl Laboratories
RMP Polyclonal Antibody
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FIGURE: 1 / 1
RMP Antibody (A301-163A) in WB
Detection of human RMP by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RMP antibody (Product # A301-163A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. RMP was also immunoprecipitated by rabbit anti-RMP antibody A301-164A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Product Details
A301-163A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 350 and 400 of Human RNA polymerase II, subunit 5 (RPB5)-mediating protein.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein. Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination. [Uniprot]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytoplasmic phosphoprotein; FLJ10575; Protein NNX3; Protein phosphatase 1 regulatory subunit 19; protein phosphatase 1, regulatory subunit 19; RNA polymerase II subunit 5-mediating protein; RPB5-mediating protein; Unconventional prefoldin RPB5 interactor 1; unnamed protein product
Gene Aliases: C19orf2; NNX3; PPP1R19; RMP; URI; URI1
UniProt ID: (Human) O94763
Entrez Gene ID: (Human) 8725
negative regulation of transcription from RNA polymerase II promoter
regulation of cell growth
regulation of transcription from RNA polymerase II promoter
response to virus
negative regulation of phosphatase activity
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
protein stabilization
cellular response to growth factor stimulus
cellular response to steroid hormone stimulus
negative regulation of intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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