Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
RNASEH2A Antibody (A304-149A) in WB
Detection of human RNASEH2A by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-RNASEH2A antibody (Product # A304-149A) (lot A304-149A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A304-149A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 249 to 299 of Human Ribonuclease H2, Subunit A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: AGS4; Aicardi-Goutieres syndrome 4 protein; Ribonuclease H2 subunit A; ribonuclease H2, large subunit; ribonuclease H2, subunit A; ribonuclease HI; Ribonuclease HI large subunit; Ribonuclease HI subunit A; ribonuclease HI, large subunit; RNase H(35); RNase H2 subunit A; RNase HI large subunit
Gene Aliases: AGS4; JUNB; RNASEH2A; RNASEHI; RNHIA; RNHL
UniProt ID: (Human) O75792
Entrez Gene ID: (Human) 10535
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support