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Product Details
PA5-58449
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human RNF111
Conjugate
Unconjugated
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2646616
Product Specific Information
Immunogen sequence: GTPSDEDNDS SFSDCLSSPS SSLHFGDSDT VTSDEDKEVS VRHSQTILNA KSRSHSARSH KWPRTETESV SGLLMKRPCL HGSSLRRLPC
Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 86%.
Target Information
The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Arkadia; E3 ubiquitin-protein ligase Arkadia; hRNF111; RING finger protein 111; RING-type E3 ubiquitin transferase Arkadia
Gene Aliases: ARK; Arkadia; RNF111
UniProt ID: (Human) Q6ZNA4, (Mouse) Q99ML9
Entrez Gene ID: (Human) 54778, (Mouse) 93836
protein polyubiquitination
multicellular organism development
pattern specification process
protein ubiquitination
positive regulation of transforming growth factor beta receptor signaling pathway
ubiquitin-dependent SMAD protein catabolic process
positive regulation of protein ubiquitination
positive regulation of transcription, DNA-templated
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of transcription from RNA polymerase II promoter
global genome nucleotide-excision repair
protein binding
zinc ion binding
ligase activity
SUMO polymer binding
SMAD binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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