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Invitrogen
RNF111 Polyclonal Antibody
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Product Details
PA5-58449
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human RNF111. Recombinant protein control fragment (Product #RP-98114).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: GTPSDEDNDS SFSDCLSSPS SSLHFGDSDT VTSDEDKEVS VRHSQTILNA KSRSHSARSH KWPRTETESV SGLLMKRPCL HGSSLRRLPC
Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 86%.
Target Information
The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Arkadia; E3 ubiquitin-protein ligase Arkadia; hRNF111; RING finger protein 111; RING-type E3 ubiquitin transferase Arkadia; unnamed protein product
Gene Aliases: ARK; Arkadia; hRNF111; RNF111
UniProt ID: (Human) Q6ZNA4, (Mouse) Q99ML9
Entrez Gene ID: (Human) 54778, (Mouse) 93836
protein binding
zinc ion binding
transferase activity
SUMO polymer binding
SMAD binding
metal ion binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
protein polyubiquitination
DNA repair
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
pattern specification process
protein ubiquitination
positive regulation of transforming growth factor beta receptor signaling pathway
positive regulation of protein ubiquitination
global genome nucleotide-excision repair
multicellular organism development
ubiquitin-dependent SMAD protein catabolic process
positive regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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