|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region of human RNF12.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 2% sucrose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||4-8 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24404 detects RNF12 in human samples.
PA1-24404 has been successfully used in Western blot and immunohistochemistry procedures.
The PA1-24404 immunogen is a synthetic peptide corresponding to a region of human RNF12.
RNF12 is a RING-H2 zinc finger protein. It has been shown to be a ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Alternatively spliced transcript variants encoding the same protein have been reported. Transcript Variant: This variant (1) lacks an exon in the 5' UTR compared to variant 2, and may represent the more abundant form. Both variants encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.