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Immunofluorescent analysis of RNF14 showing staining in the cytoplasm and nucleus of U87-MG cells. U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a RNF14 polyclonal antibody (Product # PA5-29456) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 260 of Human RNF14|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29456 targets RNF14 in WB applications and shows reactivity with Human samples.
The PA5-29456 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 260 of Human RNF14.
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
androgen receptor associated protein 54; androgen receptor-associated protein 54; ARA54; TRIAD2
ARA54; HFB30; HRIHFB2038; RNF14; TRIAD2