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Proteintech
RNF168 Polyclonal Antibody
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Product Details
21393-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
RNF168 Fusion Protein Ag16055 (221-571 aa encoded by BC033791)
Conjugate
Form
Liquid
Concentration
0.49 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: NTGDIQKYL TPKSQFGSAS HSEAVQEVRK DSVSKDIDSS DRKSPTGQDT EIEDMPTLSP QISLGVGEQG ADSSIESPMP WLCACGAEWY HEGNVKTRPS NHGKELCVLS HERPKTRVPY SKETAVMPCG RTESGCAPTS GVTQTNGNNT GETENEESCL LISKEISKRK NQESSFEAVK DPCFSAKRRK VSPESSPDQE ETEINFTQKL IDLEHLLFER HKQEEQDRLL ALQLQKEVDK EQMVPNRQKG SPDEYHLRAT SSPPDKVLNG QRKNPKDGNF KRQTHTKHPT PERGSRDKNR QVSLKMQLKQ SVNRRKMPNS TRDHCKVSKS AHSLQPSISQ KSVFQMFQRC TK (221-571 aa encoded by BC033791)
Target Information
RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with -gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF168; FLJ35794; hRNF168; RING finger protein 168; ring finger protein 168, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF168
Gene Aliases: hRNF168; RNF168
UniProt ID: (Human) Q8IYW5
Entrez Gene ID: (Human) 165918
double-strand break repair
double-strand break repair via nonhomologous end joining
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
response to ionizing radiation
protein ubiquitination
protein sumoylation
negative regulation of transcription elongation from RNA polymerase II promoter
histone H2A monoubiquitination
interstrand cross-link repair
histone H2A-K13 ubiquitination
histone H2A-K15 ubiquitination
isotype switching
positive regulation of DNA repair
protein K63-linked ubiquitination
histone H2A K63-linked ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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