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RNF168 Antibody (PA5-31119) in WB
RNF168 Polyclonal Antibody detects RNF168 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human RNF168-transfected 293T cells.7.5 % SDS-PAGE. RNF168 Polyclonal Antibody (Product # PA5-31119) dilution: 1:5,000.
Product Details
PA5-31119
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 51 and 316 of Human RNF168
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2548593
Product Specific Information
Recommended positive controls: PC-3, human RNF168-transfected 293T.
Predicted reactivity: Chimpanzee (98%), Bovine (84%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with -gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF168; FLJ35794; hRNF168; RING finger protein 168; ring finger protein 168, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF168
Gene Aliases: hRNF168; RNF168
UniProt ID: (Human) Q8IYW5
Entrez Gene ID: (Human) 165918
double-strand break repair
double-strand break repair via nonhomologous end joining
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
response to ionizing radiation
protein ubiquitination
protein sumoylation
negative regulation of transcription elongation from RNA polymerase II promoter
histone H2A monoubiquitination
interstrand cross-link repair
histone H2A-K13 ubiquitination
histone H2A-K15 ubiquitination
isotype switching
positive regulation of DNA repair
protein K63-linked ubiquitination
histone H2A K63-linked ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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