Western blot analysis of RNF168 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human RNF168-transfected 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a RNF168 polyclonal antibody (Product # PA5-31119) at a dilution of 1:5000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 51 and 316 of Human RNF168|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31119 targets RNF168 in WB applications and shows reactivity with Human samples.
The PA5-31119 immunogen is recombinant fragment corresponding to a region within amino acids 51 and 316 of Human RNF168.
The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSB repair (Stewart et al., 2009 [PubMed 19203578]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.