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Western blot analysis of HeLa Whole Cell Lysate using PA5-19597, RNF20 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19597 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Chicken, Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RNF20.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with mouse based on sequence homology.
The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SUPT6H controls estrogen receptor activity and cellular differentiation by multiple epigenomic mechanisms.
PA5-19597 was used in immunoprecipitation to study the epigenetic mechanisms by which SUPT6H regulates estrogen receptor activity
|Bedi U,Scheel AH,Hennion M,Begus-Nahrmann Y,Rüschoff J,Johnsen SA||Oncogene (34:465)||2015|
BRE1 E3 ubiquitin ligase homolog; BRE1, BRE1A, FLJ11189, FLJ20382, KIAA2779, MGC129667, MGC129668, hBRE1, BRE1 E3 ubiquitin ligase homolog; BRE1-A; E3 ubiquitin-protein ligase BRE1A; homolog of S. cerevisiae BRE1; RING finger protein 20
BRE1; BRE1A; hBRE1; RCJMB04_5a23; RNF20