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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • RNF5 Antibodies

          Invitrogen

          RNF5 Polyclonal Antibody

          1 Published Figure
          1 Reference
          View all (7) RNF5 antibodies
          Datasheet
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          Datasheet
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          Cite RNF5 Polyclonal Antibody

          • Antibody Testing Data (1)
          • Published Figures (1)
          RNF5 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          RNF5 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          RNF5 Antibody (PA5-31793) in WB

          Western Blot using RNF5 Polyclonal Antibody (Product # PA5-31793). Sample (30 µg of whole cell lysate). Lane A: 293T. 12% SDS PAGE. RNF5 Polyclonal Antibody (Product # PA5-31793) diluted at 1:1,000. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          RNF5 Antibody in Western Blot (WB)
          RNF5 Antibody in Western Blot (WB)

          Product Details

          PA5-31793

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Not Applicable

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fragment corresponding to a region within amino acids 1 and 180 of Human RNF5
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 20% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2549266

          Product Specific Information

          Recommended positive controls: 293T.

          Predicted reactivity: Mouse (97%), Rat (97%), Pig (99%), Rhesus Monkey (99%), Bovine (97%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: E3 ubiquitin-protein ligase RNF5; HsRma1; protein G16; Ram1 homolog; RING finger protein 5; ring finger protein 5, E3 ubiquitin protein ligase

          View more View less

          Gene Aliases: G16; NG2; RING5; RMA1; RNF5

          View more View less

          UniProt ID: (Human) Q99942

          View more View less

          Entrez Gene ID: (Human) 6048

          View more View less

          Function(s)
          ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity ubiquitin protein ligase activity involved in ERAD pathway ubiquitin-protein ligase
          Process(es)
          response to bacterium negative regulation of autophagy protein destabilization ERAD pathway cellular protein catabolic process protein K63-linked ubiquitination protein K48-linked ubiquitination ER-associated misfolded protein catabolic process regulation of autophagosome assembly
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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