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RNF8 Polyclonal Antibody

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Antibody Testing Data (6)

RNF8 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

RNF8 Antibody (14112-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 14112-1-AP (RNF8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RNF8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RNF8 Antibody in Immunoprecipitation (IP)

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Product Details

14112-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RNF8 Fusion Protein Ag5292 (130-485 aa encoded by BC007517)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ETIYPCLSP KNDQMIEKNK ELRTKRKFSL DELAGPGAEG PSNLKSKINK VSCESGQPVK SQGKGEVAST PSDNLDPKLT ALEPSKTTGA PIYPGFPKVT EVHHEQKASN SSASQRSLQM FKVTMSRILR LKIQMQEKHE AVMNVKKQTQ KGNSKKVVQM EQELQDLQSQ LCAEQAQQQA RVEQLEKTFQ EEEQHLQGLE IAQGEKDLKQ QLAQALQEHW ALMEELNRSK KDFEAIIQAK NKELEQTKEE KEKMQAQKEE VLSHMNDVLE NELQCIICSE YFIEAVTLNC AHSFCSYCIN EWMKRKIECP ICRKDIKSKT YSLVLDNCIN KMVNNLSSEV KERRIVLIRE RKAKRLF (130-485 aa encoded by BC007517 )

Target Information

The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.

Cell death discovery

Guo Y,Shen R,Yang K,Wang Y,Song H,Liu X,Cheng X,Wu R,Song Y,Wang D

Fri Jun 30 00:00:00 EDT 2023

RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.

Cell death discovery

Guo Y,Shen R,Yang K,Wang Y,Song H,Liu X,Cheng X,Wu R,Song Y,Wang D

Fri Jun 30 00:00:00 EDT 2023

RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.

Cell death discovery

Guo Y,Shen R,Yang K,Wang Y,Song H,Liu X,Cheng X,Wu R,Song Y,Wang D

Fri Jun 30 00:00:00 EDT 2023

SET8 Inhibition Potentiates Radiotherapy by Suppressing DNA Damage Repair in Carcinomas.

Biomedical and environmental sciences : BES

Pan D,Du YR,Li R,Shen AH,Liu XD,Li CY,Hu BR

Sun Mar 20 00:00:00 EDT 2022

Neuroprotective Effect of E3 Ubiquitin Ligase RNF8 Against Ischemic Stroke via HDAC2 Stability Reduction and Reelin-Dependent GSK3β Inhibition.

Molecular neurobiology

Zhu X,Li J,You D,Xiao Y,Huang Z,Yu W

Mon Aug 01 00:00:00 EDT 2022

Neuroprotective Effect of E3 Ubiquitin Ligase RNF8 Against Ischemic Stroke via HDAC2 Stability Reduction and Reelin-Dependent GSK3β Inhibition.

Molecular neurobiology

Zhu X,Li J,You D,Xiao Y,Huang Z,Yu W

Mon Aug 01 00:00:00 EDT 2022

53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling.

EMBO reports

Tsaridou S,Velimezi G,Willenbrock F,Chatzifrangkeskou M,Elsayed W,Panagopoulos A,Karamitros D,Gorgoulis V,Lygerou Z,Roukos V,O'Neill E,Pefani DE

Wed Aug 03 00:00:00 EDT 2022

53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling.

EMBO reports

Tsaridou S,Velimezi G,Willenbrock F,Chatzifrangkeskou M,Elsayed W,Panagopoulos A,Karamitros D,Gorgoulis V,Lygerou Z,Roukos V,O'Neill E,Pefani DE

Wed Aug 03 00:00:00 EDT 2022

RNF8 is not required for histone-to-protamine exchange in spermiogenesis†.

Biology of reproduction

Abe H,Meduri R,Li Z,Andreassen PR,Namekawa SH

Mon Nov 15 00:00:00 EST 2021

RNF8 is not required for histone-to-protamine exchange in spermiogenesis†.

Biology of reproduction

Abe H,Meduri R,Li Z,Andreassen PR,Namekawa SH

Mon Nov 15 00:00:00 EST 2021

Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.

Environmental pollution (Barking, Essex : 1987)

Liu J,Li X,Zhou G,Zhang Y,Sang Y,Wang J,Li Y,Ge W,Sun Z,Zhou X

Wed Sep 01 00:00:00 EDT 2021

RNF8-ubiquitinated KMT5A is required for RNF168-induced H2A ubiquitination in response to DNA damage.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Lu X,Xu M,Zhu Q,Zhang J,Liu G,Bao Y,Gu L,Tian Y,Wen H,Zhu WG

Thu Apr 01 00:00:00 EDT 2021

Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.

Nature communications

Hang Q,Zeng L,Wang L,Nie L,Yao F,Teng H,Deng Y,Yap S,Sun Y,Frank SJ,Chen J,Ma L

Tue Jun 29 00:00:00 EDT 2021

Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.

Nature communications

Hang Q,Zeng L,Wang L,Nie L,Yao F,Teng H,Deng Y,Yap S,Sun Y,Frank SJ,Chen J,Ma L

Tue Jun 29 00:00:00 EDT 2021

Protective Effects of Sesamin on Cytoxan-Induced Spermatogenesis Dysfunction by Regulating RNF8-ubH2A/ubH2B Pathways in Male Mice.

Frontiers in pharmacology

Hai DM,Ren JW,Chi YN,Ye RJ,Liu N,Ma L,Lan XB,Wu J,Yu JQ,Yang JM

Fri Oct 01 00:00:00 EDT 2021

RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.

Oncogenesis

Tan Q,Niu K,Zhu Y,Chen Z,Li Y,Li M,Wei D,Balajee AS,Fang H,Zhao Y

Fri Mar 05 00:00:00 EST 2021

RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.

Oncogenesis

Tan Q,Niu K,Zhu Y,Chen Z,Li Y,Li M,Wei D,Balajee AS,Fang H,Zhao Y

Fri Mar 05 00:00:00 EST 2021

BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites.

The EMBO journal

Hu Z,Mi S,Zhao T,Peng C,Peng Y,Chen L,Zhu W,Yao Y,Song Q,Li X,Li X,Jia C,Pei H

Wed Jun 17 00:00:00 EDT 2020

BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites.

The EMBO journal

Hu Z,Mi S,Zhao T,Peng C,Peng Y,Chen L,Zhu W,Yao Y,Song Q,Li X,Li X,Jia C,Pei H

Wed Jun 17 00:00:00 EDT 2020

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Journal of experimental & clinical cancer research : CR

Sun J,Zhu Z,Li W,Shen M,Cao C,Sun Q,Guo Z,Liu L,Wu D

Wed Oct 21 00:00:00 EDT 2020

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Journal of experimental & clinical cancer research : CR

Sun J,Zhu Z,Li W,Shen M,Cao C,Sun Q,Guo Z,Liu L,Wu D

Wed Oct 21 00:00:00 EDT 2020

PHF7 is a novel histone H2A E3 ligase prior to histone-to-protamine exchange during spermiogenesis.

Development (Cambridge, England)

Wang X,Kang JY,Wei L,Yang X,Sun H,Yang S,Lu L,Yan M,Bai M,Chen Y,Long J,Li N,Li D,Huang J,Lei M,Shao Z,Yuan W,Zuo E,Lu K,Liu MF,Li J

Wed Jul 10 00:00:00 EDT 2019

The deubiquitylating enzyme USP15 regulates homologous recombination repair and cancer cell response to PARP inhibitors.

Nature communications

Peng Y,Liao Q,Tan W,Peng C,Hu Z,Chen Y,Li Z,Li J,Zhen B,Zhu W,Li X,Yao Y,Song Q,Liu C,Qi X,He F,Pei H

Fri Mar 15 00:00:00 EDT 2019

L3MBTL2 regulates chromatin remodeling during spermatogenesis.

Cell death and differentiation

Meng C,Liao J,Zhao D,Huang H,Qin J,Lee TL,Chen D,Chan WY,Xia Y

Fri Nov 01 00:00:00 EDT 2019

L3MBTL2 regulates chromatin remodeling during spermatogenesis.

Cell death and differentiation

Meng C,Liao J,Zhao D,Huang H,Qin J,Lee TL,Chen D,Chan WY,Xia Y

Fri Nov 01 00:00:00 EDT 2019

GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.

Nucleic acids research

Lu X,Tang M,Zhu Q,Yang Q,Li Z,Bao Y,Liu G,Hou T,Lv Y,Zhao Y,Wang H,Yang Y,Cheng Z,Wen H,Liu B,Xu X,Gu L,Zhu WG

Mon Dec 02 00:00:00 EST 2019

The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8.

The EMBO journal

Singh AN,Oehler J,Torrecilla I,Kilgas S,Li S,Vaz B,Guérillon C,Fielden J,Hernandez-Carralero E,Cabrera E,Tullis ID,Meerang M,Barber PR,Freire R,Parsons J,Vojnovic B,Kiltie AE,Mailand N,Ramadan K

Fri Oct 04 00:00:00 EDT 2019

Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.

Cell

Gou LT,Kang JY,Dai P,Wang X,Li F,Zhao S,Zhang M,Hua MM,Lu Y,Zhu Y,Li Z,Chen H,Wu LG,Li D,Fu XD,Li J,Shi HJ,Liu MF

Thu Jun 01 00:00:00 EDT 2017

Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.

Cell

Gou LT,Kang JY,Dai P,Wang X,Li F,Zhao S,Zhang M,Hua MM,Lu Y,Zhu Y,Li Z,Chen H,Wu LG,Li D,Fu XD,Li J,Shi HJ,Liu MF

Thu Jun 01 00:00:00 EDT 2017

Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.

Cell

Gou LT,Kang JY,Dai P,Wang X,Li F,Zhao S,Zhang M,Hua MM,Lu Y,Zhu Y,Li Z,Chen H,Wu LG,Li D,Fu XD,Li J,Shi HJ,Liu MF

Thu Jun 01 00:00:00 EDT 2017

Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.

Cell

Gou LT,Kang JY,Dai P,Wang X,Li F,Zhao S,Zhang M,Hua MM,Lu Y,Zhu Y,Li Z,Chen H,Wu LG,Li D,Fu XD,Li J,Shi HJ,Liu MF

Thu Jun 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: ActA binding protein 2; ActA-interacting protein 37; AIP37; C3HC4-type zinc finger protein; E3 ubiquitin-protein ligase RNF8; FLJ12013; hRNF8; LaXp180; OTTHUMP00000039684; ring finger protein (C3HC4 type) 8; RING finger protein 8; ring finger protein 8, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF8; UBC13/UEV-interacting ring finger protein

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Gene Aliases: 3830404E21Rik; AIP37; hRNF8; KIAA0646; RNF8

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UniProt ID: (Human) O76064, (Rat) Q4KLN8, (Mouse) Q8VC56

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Entrez Gene ID: (Human) 9025, (Rat) 361815, (Mouse) 58230

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Function(s)

chromatin binding ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity ubiquitin protein ligase binding histone binding protein homodimerization activity molecular_function acid-amino acid ligase activity ubiquitin protein ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase protein modifying enzyme

Process(es)

double-strand break repair double-strand break repair via nonhomologous end joining ubiquitin-dependent protein catabolic process cellular response to DNA damage stimulus mitotic nuclear division spermatid development response to ionizing radiation histone H2A ubiquitination histone H2B ubiquitination negative regulation of transcription elongation from RNA polymerase II promoter interstrand cross-link repair histone exchange isotype switching positive regulation of DNA repair cell division protein autoubiquitination protein K63-linked ubiquitination histone H2A K63-linked ubiquitination protein K48-linked ubiquitination biological_process DNA repair cell cycle protein ubiquitination chromatin modification

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RNF8 Polyclonal Antibody

RNF8 Antibody (14112-1-AP) in ICC/IF

Product Details

14112-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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