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|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide GEPGFFVTGDRAG, corresponding to N terminal amino acids 2-14 of Human RNF8.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31259 detects RNF8 from human samples.
PA1-31259 has been successfully used in Western blot applications.
The PA1-31259 immunogen is: Synthetic peptide: GEPGFFVTGDRAG, corresponding to N terminal amino acids 2-14 of Human RNF8.
The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C3HC4-type zinc finger protein; E3 ubiquitin-protein ligase RNF8; FLJ12013; hRNF8; KIAA0646; ring finger protein (C3HC4 type) 8; RING finger protein 8; ring finger protein 8, E3 ubiquitin protein ligase; UBC13/UEV-interacting ring finger protein
hRNF8; KIAA0646; RNF8