Proteintech

RNMT Polyclonal Antibody

RNMT Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

RNMT Antibody (13743-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using RNMT antibody 13743-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

RNMT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

13743-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RNMT Fusion Protein Ag4682 (128-476 aa encoded by BC036798)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KIALEDVPE KQKNLEEGHS STVAAHYNEL QEVGLEKRSQ SRIFYLRNFN NWMKSVLIGE FLEKVRQKKK RDITVLDLGC GKGGDLLKWK KGRINKLVCT DIADVSVKQC QQRYEDMKNR RDSEYIFSAE FITADSSKEL LIDKFRDPQM CFDICSCQFV CHYSFESYEQ ADMMLRNACE RLSPGGYFIG TTPNSFELIR RLEASETESF GNEIYTVKFQ KKGDYPLFGC KYDFNLEGVV DVPEFLVYFP LLNEMAKKYN MKLVYKKTFL EFYEEKIKNN ENKMLLKRMQ ALEPYPANES SKLVSEKVDD YEHAAKYMKN SQVRLPLGTL SKSEWEATSI YLVFAFEKQQ (128-476 aa encoded by BC036798)

Target Information

RNMT belongs to the mRNA cap methyltransferase family. RNMT is a mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. It binds RNA containing 5'-terminal GpppC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.

Journal of molecular biology

Osborne MJ,Volpon L,Memarpoor-Yazdi M,Pillay S,Thambipillai A,Czarnota S,Culjkovic-Kraljacic B,Trahan C,Oeffinger M,Cowling VH,Borden KLB

Tue Mar 15 00:00:00 UTC 2022

Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.

Journal of molecular biology

Osborne MJ,Volpon L,Memarpoor-Yazdi M,Pillay S,Thambipillai A,Czarnota S,Culjkovic-Kraljacic B,Trahan C,Oeffinger M,Cowling VH,Borden KLB

Tue Mar 15 00:00:00 UTC 2022

Inhibition of cytoplasmic cap methylation identifies 5' TOP mRNAs as recapping targets and reveals recapping sites downstream of native 5' ends.

Nucleic acids research

Del Valle Morales D,Trotman JB,Bundschuh R,Schoenberg DR

Fri Apr 17 00:00:00 UTC 2020

RNA-methylation-dependent RNA processing controls the speed of the circadian clock.

Cell

Fustin JM,Doi M,Yamaguchi Y,Hida H,Nishimura S,Yoshida M,Isagawa T,Morioka MS,Kakeya H,Manabe I,Okamura H

Thu Nov 07 00:00:00 UTC 2013

RNA-methylation-dependent RNA processing controls the speed of the circadian clock.

Cell

Fustin JM,Doi M,Yamaguchi Y,Hida H,Nishimura S,Yoshida M,Isagawa T,Morioka MS,Kakeya H,Manabe I,Okamura H

Thu Nov 07 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: hcm1p; hCMT1; mRNA (guanine-7-)methyltransferase; mRNA (guanine-N(7))-methyltransferase; mRNA (guanine-N(7)-)-methyltransferase; mRNA cap guanine-N(7) methyltransferase; mRNA cap guanine-N7 methyltransferase; mRNA cap methyltransferase; RG7MT1; RNA (guanine-7-) methyltransferase

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Gene Aliases: 2610002P10Rik; AI848273; cm1p; CMT1; CMT1c; hCMT1; hMet; KIAA0398; MET; mKIAA0398; RG7MT1; RNMT

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UniProt ID: (Human) O43148, (Mouse) Q9D0L8

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Entrez Gene ID: (Human) 8731, (Mouse) 67897

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Function(s)

RNA binding mRNA (guanine-N7-)-methyltransferase activity protein binding methyltransferase activity transferase activity RNA methyltransferase

Process(es)

transcription from RNA polymerase II promoter 7-methylguanosine mRNA capping RNA (guanine-N7)-methylation mRNA processing methylation mRNA methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RNMT Polyclonal Antibody

RNMT Antibody (13743-1-AP) in ICC/IF

Product Details

13743-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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