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Product Details
PA5-120344
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1452-1651 of human ROBO1 (NP_002932.1).
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913916
Product Specific Information
Positive test controls include: 293T, U-251MG, HeLa, 293T, U-251MG, HeLa, Mouse brain, Rat brain. The target is usually found in the following locations: Cell membrane, Cell projection, Single-pass type I membrane protein, axon.
Immunogen sequence: AAVMQKTRPA KKLKHQPGHL RRETYTDDLP PPPVPPPAIK SPTAQSKTQL EVRPVVVPKL PSMDARTDRS SDRKGSSYKG REVLDGRQVV DMRTNPGDPR EAQEQQNDGK GRGNKAAKRD LPPAKTHLIQ EDILPYCRPT FPTSNNPRDP SSSSSMSSRG SGSRQREQAN VGRRNIAEMQ VLGGYERGED NNEELEETES
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deleted in U twenty twenty; FLJ21882; H-Robo-1; HGNC:10249; MGC131599; MGC133277; roundabout 1; Roundabout homolog 1; roundabout, axon guidance receptor, homolog 1
Gene Aliases: AW494633; AW742721; DUTT1; Gm310; ROBO1; SAX3
UniProt ID: (Human) Q9Y6N7, (Rat) O55005, (Mouse) O89026
Entrez Gene ID: (Human) 6091, (Rat) 58946, (Mouse) 19876
cell migration involved in sprouting angiogenesis
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
nervous system development
axon guidance
heart development
axon midline choice point recognition
chemorepulsion involved in postnatal olfactory bulb interneuron migration
negative regulation of cell migration
negative regulation of mammary gland epithelial cell proliferation
positive regulation of Rho protein signal transduction
Roundabout signaling pathway
positive regulation of axonogenesis
negative regulation of negative chemotaxis
negative regulation of chemokine-mediated signaling pathway
central nervous system development
negative regulation of cell proliferation
spinal cord development
olfactory bulb interneuron development
regulation of dendrite morphogenesis
mammary duct terminal end bud growth
chemotaxis
multicellular organism development
cell differentiation
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