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Invitrogen
ROBO1 Polyclonal Antibody
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FIGURE: 1 / 5
ROBO1 Antibody (PA5-29917) in ICC/IF
ROBO1 Polyclonal Antibody detects ROBO1 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ROBO1 stained by ROBO1 Polyclonal Antibody (Product # PA5-29917) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Product Details
PA5-29917
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human ROBO1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.26 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, MDA-Mb-361, HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1).
Predicted reactivity: Mouse (100%), Rat (97%), Zebrafish (80%), Xenopus laevis (95%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Deleted in U twenty twenty; FLJ21882; H-Robo-1; HGNC:10249; MGC131599; MGC133277; roundabout 1; Roundabout homolog 1; roundabout, axon guidance receptor, homolog 1; Roundabout1 protein
Gene Aliases: AW494633; AW742721; DUTT1; Gm310; ROBO1; SAX3
UniProt ID: (Human) Q9Y6N7, (Mouse) O89026
Entrez Gene ID: (Human) 6091, (Chicken) 395292, (Mouse) 19876
cell migration involved in sprouting angiogenesis
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
nervous system development
axon guidance
heart development
axon midline choice point recognition
chemorepulsion involved in postnatal olfactory bulb interneuron migration
negative regulation of cell migration
negative regulation of mammary gland epithelial cell proliferation
positive regulation of Rho protein signal transduction
Roundabout signaling pathway
positive regulation of axonogenesis
negative regulation of negative chemotaxis
negative regulation of chemokine-mediated signaling pathway
chemotaxis
multicellular organism development
negative regulation of cell proliferation
olfactory bulb interneuron development
cell differentiation
regulation of dendrite morphogenesis
mammary duct terminal end bud growth
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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