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Invitrogen
ROBO1 Polyclonal Antibody
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FIGURE: 1 / 2
ROBO1 Antibody (PA5-47196) in IHC (F)
Immunohistochemical analysis of ROBO1 in perfusion fixed frozen sections of rat brain. Samples were incubated in ROBO1 polyclonal antibody (Product # PA5-47196) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and ... View More
Product Details
PA5-47196
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat ROBO1 Lys19-Ile560
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ21882; HGNC:10249; MGC131599; MGC133277; roundabout 1; Roundabout homolog 1
Gene Aliases: Robo1
UniProt ID: (Rat) O55005
Entrez Gene ID: (Rat) 58946
cell migration involved in sprouting angiogenesis
activation of cysteine-type endopeptidase activity involved in apoptotic process
homophilic cell adhesion via plasma membrane adhesion molecules
axon guidance
central nervous system development
negative regulation of cell proliferation
axon midline choice point recognition
spinal cord development
chemorepulsion involved in postnatal olfactory bulb interneuron migration
olfactory bulb interneuron development
negative regulation of mammary gland epithelial cell proliferation
Roundabout signaling pathway
regulation of dendrite morphogenesis
positive regulation of axonogenesis
negative regulation of negative chemotaxis
mammary duct terminal end bud growth
negative regulation of chemokine-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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