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Invitrogen
ROBO1 Recombinant Mouse Monoclonal Antibody (10E2-R)
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FIGURE: 1 / 3
ROBO1 Antibody (MA5-50631) in IHC (P)
Immunohistochemistry of ROBO1 in paraffin-embedded human placenta tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with ROBO1 recombinant monoclonal antibody (Product # MA5-50631) with a dilution of 1:200 for 1 hour at room ... View More
Product Details
MA5-50631
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10E2-R
Immunogen
Recombinant protein within Human ROBO1 aa 51-300.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: MCF-7 cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, PC-12 cell lysate, RAW264.7 cell lysate, human breast tissue, human placenta tissue.
Predicted band size: 181 kDa
Subcellular Location: Cell membrane, Cell projection, axon, Endoplasmic reticulum-Golgi intermediate compartment membrane.
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deleted in U twenty twenty; FLJ21882; H-Robo-1; HGNC:10249; MGC131599; MGC133277; roundabout 1; Roundabout homolog 1; roundabout, axon guidance receptor, homolog 1
Gene Aliases: AW494633; AW742721; DUTT1; Gm310; ROBO1; SAX3
UniProt ID: (Human) Q9Y6N7, (Rat) O55005, (Mouse) O89026
Entrez Gene ID: (Human) 6091, (Rat) 58946, (Mouse) 19876
cell migration involved in sprouting angiogenesis
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
nervous system development
axon guidance
heart development
axon midline choice point recognition
chemorepulsion involved in postnatal olfactory bulb interneuron migration
negative regulation of cell migration
negative regulation of mammary gland epithelial cell proliferation
positive regulation of Rho protein signal transduction
Roundabout signaling pathway
positive regulation of axonogenesis
negative regulation of negative chemotaxis
negative regulation of chemokine-mediated signaling pathway
central nervous system development
negative regulation of cell proliferation
spinal cord development
olfactory bulb interneuron development
regulation of dendrite morphogenesis
mammary duct terminal end bud growth
chemotaxis
multicellular organism development
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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