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Invitrogen
ROBO3 Polyclonal Antibody
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FIGURE: 1 / 1
ROBO3 Antibody (PA5-92319) in WB
Western blot analysis of ROBO3 in mouse brain. Samples were incubated with ROBO3 Polyclonal antibody (Product # PA5-92319) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-92319
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1237-1386 of human ROBO3 (NP_0717652)
Conjugate
Form
Liquid
Concentration
0.64 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: EMTPPLQGPR ARFRKKPKAL PYRRENSPGD LPPPPLPPPE EEASWALELR AAGSMSSLER ERSGERKAVQ AVPLAAQRVL HPDEEAWLPY SRPSFLSRGQ GTSTCSTAGS NSSRGSSSSR GSRGPGRSRS RSQSRSQSQR PGQKRREEPR; Positive Samples: Mouse brain; Cellular Location: Membrane, Single-pass type I membrane protein
Target Information
This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ21044; retinoblastoma inhibiting gene 1; Retinoblastoma-inhibiting gene 1 protein; Rig-1; Roundabout guidance receptor 3; Roundabout homolog 3
Gene Aliases: Rbig1; Rig-1; Rig1; Robo3; Robo3a; Robo3b
UniProt ID: (Mouse) Q9Z2I4
Entrez Gene ID: (Mouse) 19649
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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