Invitrogen

ROCK2 Polyclonal Antibody

Advanced Verification
4 Published Figures
2 References
View all (40) ROCK2 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-78290

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Immunoprecipitation (IP)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2736534

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, Rat2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, A549, H1299, HCT116

Predicted Reactivity: Xenopus laevis (80%), Rhesus Monkey (100%), Bovine (97%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases. ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: p150 ROK-alpha; p164 ROCK-2; Rho kinase 2; Rho-associated coiled-coil forming kinage 2; Rho-associated coiled-coil forming kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; RhoA - binding serine/threosine kinase alpha (ROK - alpha); RhoA-binding kinase 2; ROCK II; ROCK-II; rockii; ROKalpha

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Gene Aliases: B230113H15Rik; KIAA0619; mKIAA0619; Rho-kinase; ROCK-II; ROCK2; Rock2m; ROK; ROKalpha

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UniProt ID: (Human) O75116, (Rat) Q62868, (Mouse) P70336

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Entrez Gene ID: (Human) 9475, (Rat) 25537, (Mouse) 19878

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Function(s)
protein serine/threonine kinase activity structural molecule activity protein binding ATP binding Rho GTPase binding poly(A) RNA binding metal ion binding Rho-dependent protein serine/threonine kinase activity kinase activity nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
cytokinesis positive regulation of protein phosphorylation protein phosphorylation smooth muscle contraction I-kappaB kinase/NF-kappaB signaling Rho protein signal transduction positive regulation of endothelial cell migration positive regulation of gene expression positive regulation of centrosome duplication negative regulation of angiogenesis regulation of cell adhesion cortical actin cytoskeleton organization regulation of actin cytoskeleton organization negative regulation of myosin-light-chain-phosphatase activity regulation of circadian rhythm regulation of keratinocyte differentiation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway rhythmic process centrosome duplication regulation of stress fiber assembly regulation of focal adhesion assembly cellular response to testosterone stimulus establishment of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly regulation of establishment of cell polarity regulation of cell motility neural tube closure extrinsic apoptotic signaling pathway via death domain receptors actin cytoskeleton organization chromosome condensation dendrite morphogenesis phosphorylation intracellular signal transduction
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