Invitrogen

ROCK2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (39) ROCK2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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ROCK2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of ROCK2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ROCK2 Rabbit Polyclonal Antibody (Product # PA5-78290) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ROCK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — ROCK2 Polyclonal Antibody detects ROCK2 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ROCK2 Polyclonal Antibody (Product # PA5-78290) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

ROCK2 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — ROCK2 Polyclonal Antibody detects ROCK2 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: ROCK2 protein stained by ROCK2 Polyclonal Antibody (Product # PA5-78290) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.

ROCK2 Antibody in Western Blot (WB) — Western blot was performed using Anti-ROCK2 Polyclonal Antibody (Product # PA5-78290) and a 180 kDa corresponding to ROCK2 along with an uncharacterized band around 53 kDa was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), U-87 MG (Lane 5) and C1C12 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (1:3000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

ROCK2 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of target protein. 293T cells were transfected with ROCK2 shRNA and decrease in signal intensity was observed in Western blotting using Anti-ROCK2 Polyclonal Antibody (Product # PA5-78290). {KD}

Product Details

PA5-78290

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.67 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2736534

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, Rat2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, A549, H1299, HCT116

Predicted Reactivity: Xenopus laevis (80%), Rhesus Monkey (100%), Bovine (97%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lamin A and the LINC complex act as potential tumor suppressors in Ewing Sarcoma.

Cell death & disease

Chiarini F,Paganelli F,Balestra T,Capanni C,Fazio A,Manara MC,Landuzzi L,Petrini S,Evangelisti C,Lollini PL,Martelli AM,Lattanzi G,Scotlandi K

Published figure using ROCK2 polyclonal antibody (Product # PA5-78290) in Western Blot

Thu Apr 14 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: p150 ROK-alpha; p164 ROCK-2; Rho kinase 2; Rho-associated coiled-coil forming kinage 2; Rho-associated coiled-coil forming kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; RhoA - binding serine/threosine kinase alpha (ROK - alpha); RhoA-binding kinase 2; ROCK II; ROCK-II; rockii; ROKalpha

Gene Aliases: B230113H15Rik; KIAA0619; mKIAA0619; Rho-kinase; ROCK-II; ROCK2; Rock2m; ROK; ROKalpha

UniProt ID: (Human) O75116, (Rat) Q62868, (Mouse) P70336

Entrez Gene ID: (Human) 9475, (Rat) 25537, (Mouse) 19878

Molecular Function: non-receptor serine/threonine protein kinase