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Invitrogen

ROCK2 Polyclonal Antibody

Advanced Verification
4 Published Figures
2 References
View all (42) ROCK2 antibodies

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Product Details

PA5-78290

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Immunoprecipitation (IP)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2736534

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, Rat2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, A549, H1299, HCT116

Predicted Reactivity: Xenopus laevis (80%), Rhesus Monkey (100%), Bovine (97%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases. ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: p150 ROK-alpha; p164 ROCK-2; Rho kinase 2; Rho-associated coiled-coil forming kinage 2; Rho-associated coiled-coil forming kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; RhoA - binding serine/threosine kinase alpha (ROK - alpha); RhoA-binding kinase 2; ROCK II; ROCK-II; rockii; ROKalpha; unnamed protein product

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Gene Aliases: B230113H15Rik; KIAA0619; mKIAA0619; Rho-kinase; ROCK-II; ROCK2; Rock2m; ROK; ROKalpha

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UniProt ID: (Human) O75116, (Rat) Q62868, (Mouse) P70336

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Entrez Gene ID: (Human) 9475, (Rat) 25537, (Mouse) 19878

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Function(s)
nucleotide binding protease binding RNA binding protein kinase activity protein serine/threonine kinase activity structural molecule activity protein binding ATP binding zinc ion binding kinase activity transferase activity small GTPase binding metal ion binding tau protein binding tau-protein kinase activity endopeptidase activator activity Rho-dependent protein serine/threonine kinase activity protein serine kinase activity Rho GTPase binding poly(A) RNA binding transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
mitotic cytokinesis epithelial to mesenchymal transition response to ischemia aortic valve morphogenesis protein phosphorylation smooth muscle contraction signal transduction Rho protein signal transduction positive regulation of cardiac muscle hypertrophy positive regulation of gene expression negative regulation of gene expression positive regulation of centrosome duplication negative regulation of angiogenesis actin cytoskeleton organization regulation of cell adhesion positive regulation of cell migration cortical actin cytoskeleton organization actomyosin structure organization regulation of neurological system process positive regulation of connective tissue growth factor production regulation of actin cytoskeleton organization regulation of circadian rhythm stress fiber assembly positive regulation of MAPK cascade modulation by host of viral process negative regulation of nitric oxide biosynthetic process regulation of keratinocyte differentiation positive regulation of phosphate metabolic process rhythmic process embryonic morphogenesis centrosome duplication regulation of stress fiber assembly positive regulation of stress fiber assembly regulation of focal adhesion assembly mRNA destabilization negative regulation of biomineral tissue development response to transforming growth factor beta protein localization to plasma membrane positive regulation of fibroblast growth factor production regulation of blood vessel diameter regulation of angiotensin-activated signaling pathway negative regulation of protein localization to lysosome regulation of cellular response to hypoxia regulation of cell junction assembly regulation of beta-amyloid formation positive regulation of beta-amyloid formation positive regulation of protein localization to early endosome positive regulation of amyloid precursor protein catabolic process regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly cellular response to acetylcholine positive regulation of connective tissue replacement response to angiotensin regulation of establishment of cell polarity regulation of cell motility neural tube closure extrinsic apoptotic signaling pathway via death domain receptors chromosome condensation dendrite morphogenesis phosphorylation intracellular signal transduction
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