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ROCK2 Monoclonal Antibody (1E12)

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ROCK2 Monoclonal Antibody (1E12)

Product Details

H00009475-M02

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1.5 µg/mL
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

ELISA (ELISA)

0.3 ng/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1E12

Immunogen

ROCK2 (NP_004841, 1279 a.a. approximately 1388 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: KPLWHMFKPP PALECRRCHI KCHKDHMDKK EEIIAPCKVY YDISTAKNLL LLANSTEEQQ KWVSRLVKKI PKKPPAPDPF ARSSPRTSMK IQQNQSIRRP SRQLAPNKPS

Target Information

ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases. ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II; ROCK II; rockii; unnamed protein product

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Gene Aliases: ROCK-II

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UniProt ID: (Human) O75116

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Entrez Gene ID: (Human) 9475

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Function(s)
protease binding RNA binding protein kinase activity protein serine/threonine kinase activity structural molecule activity protein binding ATP binding small GTPase binding tau protein binding tau-protein kinase activity endopeptidase activator activity Rho-dependent protein serine/threonine kinase activity protein serine kinase activity
Process(es)
mitotic cytokinesis epithelial to mesenchymal transition response to ischemia aortic valve morphogenesis protein phosphorylation smooth muscle contraction signal transduction Rho protein signal transduction positive regulation of cardiac muscle hypertrophy positive regulation of gene expression negative regulation of gene expression positive regulation of centrosome duplication negative regulation of angiogenesis actin cytoskeleton organization regulation of cell adhesion positive regulation of cell migration cortical actin cytoskeleton organization actomyosin structure organization regulation of neurological system process positive regulation of connective tissue growth factor production regulation of actin cytoskeleton organization regulation of circadian rhythm positive regulation of MAPK cascade modulation by host of viral process negative regulation of nitric oxide biosynthetic process regulation of keratinocyte differentiation embryonic morphogenesis centrosome duplication regulation of stress fiber assembly positive regulation of stress fiber assembly regulation of focal adhesion assembly mRNA destabilization negative regulation of biomineral tissue development response to transforming growth factor beta protein localization to plasma membrane positive regulation of fibroblast growth factor production regulation of blood vessel diameter regulation of angiotensin-activated signaling pathway negative regulation of protein localization to lysosome regulation of cellular response to hypoxia regulation of cell junction assembly regulation of beta-amyloid formation positive regulation of beta-amyloid formation positive regulation of protein localization to early endosome positive regulation of amyloid precursor protein catabolic process regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly cellular response to acetylcholine positive regulation of connective tissue replacement response to angiotensin regulation of establishment of cell polarity regulation of cell motility
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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