Proteintech

ROCK2(middle) Polyclonal Antibody

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ROCK2(middle) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 21645-1-AP (ROCK2 (middle) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ROCK2(middle) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 21645-1-AP (ROCK2 (middle) antibody) at dilution of 1:100 (under 40x lens).

ROCK2(middle) Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2 (middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ROCK2(middle) Antibody in Immunoprecipitation (IP) — IP result of anti-ROCK2 (middle) (IP:21645-1-AP, 5ug; Detection:21645-1-AP 1:700) with HeLa cells lysate 1320ug.

Product Details

21645-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ROCK2(middle) Fusion Protein Ag16330 (1-181 aa encoded by BC111801) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.52 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is specific to ROCK2.

Immunogen sequence: MEIDMTYQL KVIQQSLEQE EAEHKATKAR LADKNKIYES IEEAKSEAMK EMEKKLLEER TLKQKVENLL LEAEKRCSLL DCDLKQSQQK INELLKQKDV LNEDVRNLTL KIEQETQKRC LTQNDLKMQT QQVNTLKMSE KQLKQENNHL MEMKMNLEKQ NAELRKERQD ADGQMKELQD QL (1-181 aa encoded by BC111801)

Target Information

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Megakaryocytic Smad4 Regulates Platelet Function through Syk and ROCK2 Expression.

Molecular pharmacology

Wang Y,Jiang L,Mo X,Lan Y,Yang X,Liu X,Zhang J,Zhu L,Liu J,Wu X

Fri Sep 01 00:00:00 EDT 2017

FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.

The journal of sexual medicine

Cui K,Ruan Y,Wang T,Rao K,Chen Z,Wang S,Liu J

Wed Mar 01 00:00:00 EST 2017

Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.

Oncotarget

Ching RH,Lau EY,Ling PM,Lee JM,Ma MK,Cheng BY,Lo RC,Ng IO,Lee TK

Tue Dec 22 00:00:00 EST 2015

Rock2 promotes the invasion and metastasis of hepatocellular carcinoma by modifying MMP2 ubiquitination and degradation.

Biochemical and biophysical research communications

Huang D,Du X,Yuan R,Chen L,Liu T,Wen C,Huang M,Li M,Hao L,Shao J

Fri Oct 10 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: p150 ROK-alpha; p164 ROCK-2; Rho kinase 2; Rho-associated coiled-coil forming kinage 2; Rho-associated coiled-coil forming kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; RhoA - binding serine/threosine kinase alpha (ROK - alpha); RhoA-binding kinase 2; ROCK II; ROCK-II; rockii; ROKalpha

Gene Aliases: B230113H15Rik; KIAA0619; mKIAA0619; Rho-kinase; ROCK-II; ROCK2; Rock2m; ROK; ROKalpha

UniProt ID: (Human) O75116, (Rat) Q62868, (Mouse) P70336

Entrez Gene ID: (Human) 9475, (Rat) 25537, (Mouse) 19878

Molecular Function: non-receptor serine/threonine protein kinase