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Invitrogen
RORA Polyclonal Antibody
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FIGURE: 1 / 7
RORA Antibody (PA5-11224) in ICC/IF
Immunocytochemistry analysis of RORA in HeLa cells. Samples were incubated with RORA polyclonal antibody (Product # PA5-11224) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). RORA im... View More
Product Details
PA5-11224
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 193-222 amino acids from human RORA
Conjugate
Form
Liquid
Concentration
2.0 mg/mL
Amount
800 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
RORA is a receptor for retinoic acid belonging to the NR1 subfamily of nuclear hormone receptors with a nuclear receptor DNA binding domain. RORA binds either as a monomer or as a homodimer to the retinoic acid response element and thus regulates gene expression and also controls cell function. Though the exact function of RORA is yet unknown, it interacts with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, NM23-1, the product of a tumor metastasis suppressor candidate gene, CDK7, NCOA3 and NCOA6 coactivators. It is found to express in mesenchymal stem cells derived from bone marrow and are known to act by direct modulation of a bone matrix component. It is widely expressed in most of the tissues. Chromosomal aberrations involving RORA may be a cause of acute promyelocytic leukemia (APL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; putative new variant; RAR-related orphan nuclear receptor alpha; RAR-related orphan receptor A; retinoic acid receptor-related orphan receptor alpha; retinoid-related orphan receptor alpha; Retinoid-related orphan receptor-alpha; ROR Alpha; ROR-alpha; Roralpha; transcription factor RZR-alpha
Gene Aliases: IDDECA; NR1F1; ROR1; ROR2; ROR3; RORA; RORa1; RORalpha; RZR-ALPHA; RZRA
UniProt ID: (Human) P35398
Entrez Gene ID: (Human) 6095
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
transcription corepressor binding
transcription coactivator binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
beta-catenin binding
oxysterol binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
transcription factor activity, direct ligand regulated sequence-specific DNA binding
angiogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
xenobiotic metabolic process
nitric oxide biosynthetic process
circadian rhythm
regulation of smoothened signaling pathway
regulation of gene expression
positive regulation of vascular endothelial growth factor production
regulation of glucose metabolic process
regulation of steroid metabolic process
cerebellar Purkinje cell differentiation
cerebellar granule cell precursor proliferation
intracellular receptor signaling pathway
circadian regulation of gene expression
cellular response to sterol
cholesterol homeostasis
muscle cell differentiation
regulation of circadian rhythm
positive regulation of circadian rhythm
regulation of macrophage activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cGMP metabolic process
rhythmic process
negative regulation of inflammatory response
triglyceride homeostasis
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to hypoxia
T-helper 17 cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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