Proteintech

RPA2 Polyclonal Antibody

RPA2 Antibody in Immunocytochemistry (ICC/IF)

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RPA2 Antibody (10412-1-AP) in ICC/IF

Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10412-1-AP (RPA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

RPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RPA2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10412-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RPA2 Fusion Protein Ag0690 (80-270 aa encoded by BC001630)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: IRHAEKAPT NIVYKIDDMT AAPMDVRQWV DTDDTSSENT VVPPETYVKV AGHLRSFQNK KSLVAFKIMP LEDMNEFTTH ILEVINAHMV LSKANSQPSA GRAPISNPGM SEAGNFGGNS FMPANGLTVA QNQVLNLIKA CPRPEGLNFQ DLKNQLKHMS VSSIKQAVDF LSNEGHIYST VDDDHFKSTD AE (80-270 aa encoded by BC001630)

Target Information

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NKAP acts with HDAC3 to prevent R-loop associated genome instability.

Cell death and differentiation

Zhang X,Duan J,Li Y,Jin X,Wu C,Yang X,Lu W,Ge W

Sat Jul 01 00:00:00 UTC 2023

Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice.

Nature communications

Jiang X,Cheng Y,Zhu Y,Xu C,Li Q,Xing X,Li W,Zou J,Meng L,Azhar M,Cao Y,Tong X,Qin W,Zhu X,Bao J

Thu Jun 22 00:00:00 UTC 2023

REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.

Cell death & disease

Chen Y,Jie X,Xing B,Wu Z,Yang X,Rao X,Xu Y,Zhou D,Dong X,Zhang T,Yang K,Li Z,Wu G

Thu Feb 03 00:00:00 UTC 2022

The ZFP541-KCTD19 complex is essential for pachytene progression by activating meiotic genes during mouse spermatogenesis.

Journal of genetics and genomics = Yi chuan xue bao

Li Y,Meng R,Li S,Gu B,Xu X,Zhang H,Tan X,Shao T,Wang J,Xu D,Wang F

Tue Nov 01 00:00:00 UTC 2022

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Biology

Gourdomichali O,Zonke K,Kattan FG,Makridakis M,Kontostathi G,Vlahou A,Doxakis E

Fri Feb 11 00:00:00 UTC 2022

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.

Molecular cell

Zhao J,Tian S,Guo Q,Bao K,Yu G,Wang X,Shen X,Zhang J,Chen J,Yang Y,Liu L,Li X,Hao J,Yang N,Liu Z,Ai D,Yang J,Zhu Y,Yao Z,Ma S,Zhang K,Shi L

Thu Jul 21 00:00:00 UTC 2022

Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics.

Cancer letters

Ma S,Zhang J,Guo Q,Cao C,Bao K,Liu L,Chen CD,Liu Z,Yang J,Yang N,Yao Z,Shi L

Fri Apr 01 00:00:00 UTC 2022

Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics.

Cancer letters

Ma S,Zhang J,Guo Q,Cao C,Bao K,Liu L,Chen CD,Liu Z,Yang J,Yang N,Yao Z,Shi L

Fri Apr 01 00:00:00 UTC 2022

Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics.

Cancer letters

Ma S,Zhang J,Guo Q,Cao C,Bao K,Liu L,Chen CD,Liu Z,Yang J,Yang N,Yao Z,Shi L

Fri Apr 01 00:00:00 UTC 2022

Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin.

Nature communications

Zhang T,Yang H,Zhou Z,Bai Y,Wang J,Wang W

Thu Sep 01 00:00:00 UTC 2022

Acquired temozolomide resistance in MGMT^low gliomas is associated with regulation of homologous recombination repair by ROCK2.

Cell death & disease

Zhang X,Li T,Yang M,Du Q,Wang R,Fu B,Tan Y,Cao M,Chen Y,Wang Q,Hu R

Thu Feb 10 00:00:00 UTC 2022

Acquired temozolomide resistance in MGMT^low gliomas is associated with regulation of homologous recombination repair by ROCK2.

Cell death & disease

Zhang X,Li T,Yang M,Du Q,Wang R,Fu B,Tan Y,Cao M,Chen Y,Wang Q,Hu R

Thu Feb 10 00:00:00 UTC 2022

C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex.

Molecular cell

Bai Y,Wang W,Li S,Zhan J,Li H,Zhao M,Zhou XA,Li S,Li X,Huo Y,Shen Q,Zhou M,Zhang H,Luo J,Sung P,Zhu WG,Xu X,Wang J

Thu Sep 19 00:00:00 UTC 2019

A methylation-phosphorylation switch determines Plk1 kinase activity and function in DNA damage repair.

Science advances

Li W,Wang HY,Zhao X,Duan H,Cheng B,Liu Y,Zhao M,Shu W,Mei Y,Wen Z,Tang M,Guo L,Li G,Chen Q,Liu X,Du HN

Fri Mar 01 00:00:00 UTC 2019

5-Azacytidine enhances the radiosensitivity of CNE2 and SUNE1 cells in vitro and in vivo possibly by altering DNA methylation.

PloS one

Jiang W,Li YQ,Liu N,Sun Y,He QM,Jiang N,Xu YF,Chen L,Ma J

Thu Dec 17 00:00:00 UTC 2015

5-Azacytidine enhances the radiosensitivity of CNE2 and SUNE1 cells in vitro and in vivo possibly by altering DNA methylation.

PloS one

Jiang W,Li YQ,Liu N,Sun Y,He QM,Jiang N,Xu YF,Chen L,Ma J

Thu Dec 17 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: 30-kDa protein; HSSB; p32; p32-subunit of replication protein A; p34; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A 34kDa subunit; replication protein A2, 32kDa; RF-A protein 2; RFA; RP-A p32; RP-A p34; RP21C; RP4-547C9.3; RpLP1; RpP2

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Gene Aliases: AA409079; AI325195; AU020965; REPA2; Rf-A2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34

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UniProt ID: (Human) P15927, (Mouse) Q62193, (Rat) Q63528

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Entrez Gene ID: (Human) 6118, (Mouse) 19891, (Rat) 59102

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Function(s)

damaged DNA binding single-stranded DNA binding protein binding enzyme binding protein phosphatase binding ubiquitin protein ligase binding protein N-terminus binding DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RPA2 Polyclonal Antibody

RPA2 Antibody (10412-1-AP) in ICC/IF

Product Details

10412-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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