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RPA2 Polyclonal Antibody

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RPA2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10412-1-AP (RPA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

RPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10412-1-Ap (RPA2 antibody) at dilution of 1:100 (under 10x lens).

RPA2 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10412-1-AP (RPA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RPA2 Antibody in Immunoprecipitation (IP) — IP result of anti-RPA2 (IP:10412-1-Ap, 4ug; Detection:10412-1-Ap 1:1000) with mouse kidney tissue lysate 4000ug.

Product Details

10412-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RPA2 Fusion Protein Ag0690 (80-270 aa encoded by BC001630)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: IRHAEKAPT NIVYKIDDMT AAPMDVRQWV DTDDTSSENT VVPPETYVKV AGHLRSFQNK KSLVAFKIMP LEDMNEFTTH ILEVINAHMV LSKANSQPSA GRAPISNPGM SEAGNFGGNS FMPANGLTVA QNQVLNLIKA CPRPEGLNFQ DLKNQLKHMS VSSIKQAVDF LSNEGHIYST VDDDHFKSTD AE (80-270 aa encoded by BC001630)

Target Information

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Nature communications

Zhang T,Yang H,Zhou Z,Bai Y,Wang J,Wang W

Thu Sep 01 00:00:00 EDT 2022

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.

Molecular cell

Zhao J,Tian S,Guo Q,Bao K,Yu G,Wang X,Shen X,Zhang J,Chen J,Yang Y,Liu L,Li X,Hao J,Yang N,Liu Z,Ai D,Yang J,Zhu Y,Yao Z,Ma S,Zhang K,Shi L

Thu Jul 21 00:00:00 EDT 2022

Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics.

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Ma S,Zhang J,Guo Q,Cao C,Bao K,Liu L,Chen CD,Liu Z,Yang J,Yang N,Yao Z,Shi L

Fri Apr 01 00:00:00 EDT 2022

Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics.

Cancer letters

Ma S,Zhang J,Guo Q,Cao C,Bao K,Liu L,Chen CD,Liu Z,Yang J,Yang N,Yao Z,Shi L

Fri Apr 01 00:00:00 EDT 2022

Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics.

Cancer letters

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Fri Apr 01 00:00:00 EDT 2022

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Biology

Gourdomichali O,Zonke K,Kattan FG,Makridakis M,Kontostathi G,Vlahou A,Doxakis E

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Acquired temozolomide resistance in MGMT^low gliomas is associated with regulation of homologous recombination repair by ROCK2.

Cell death & disease

Zhang X,Li T,Yang M,Du Q,Wang R,Fu B,Tan Y,Cao M,Chen Y,Wang Q,Hu R

Thu Feb 10 00:00:00 EST 2022

Acquired temozolomide resistance in MGMT^low gliomas is associated with regulation of homologous recombination repair by ROCK2.

Cell death & disease

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REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.

Cell death & disease

Chen Y,Jie X,Xing B,Wu Z,Yang X,Rao X,Xu Y,Zhou D,Dong X,Zhang T,Yang K,Li Z,Wu G

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C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex.

Molecular cell

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Thu Sep 19 00:00:00 EDT 2019

A methylation-phosphorylation switch determines Plk1 kinase activity and function in DNA damage repair.

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5-Azacytidine enhances the radiosensitivity of CNE2 and SUNE1 cells in vitro and in vivo possibly by altering DNA methylation.

PloS one

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5-Azacytidine enhances the radiosensitivity of CNE2 and SUNE1 cells in vitro and in vivo possibly by altering DNA methylation.

PloS one

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Bioinformatics

Protein Aliases: 30-kDa protein; HSSB; p32-subunit of replication protein A; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A 34kDa subunit; replication protein A2, 32kDa; RF-A protein 2; RFA; RP-A p32; RP-A p34; RP4-547C9.3; RpLP1; RpP2

Gene Aliases: AA409079; AI325195; AU020965; REPA2; Rf-A2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34

UniProt ID: (Human) P15927, (Rat) Q63528

Entrez Gene ID: (Human) 6118, (Mouse) 19891, (Rat) 59102

Function(s)

Process(es)

damaged DNA binding single-stranded DNA binding protein binding enzyme binding protein phosphatase binding ubiquitin protein ligase binding protein N-terminus binding DNA binding

RPA2 Polyclonal Antibody

Product Details

10412-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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