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RPAP1 Polyclonal Antibody

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RPAP1 Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 15138-1-AP (RPAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

RPAP1 Antibody in Immunoprecipitation (IP) — IP result of anti-RPAP1 (IP:15138-1-AP, 3ug; Detection:15138-1-AP 1:800) with HeLa cells lysate 3000ug.

Product Details

15138-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPAP1 Fusion Protein Ag7286 (1-351 aa encoded by BC000246) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.17 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MLSRPKPGE SEVDLLHFQS QFLAAGAAPA VQLVKKGNRG GGDANSDRPP LQDHRDVVML DNLPDLPPAL VPSPPKRARP SPGHCLPEDE DPEERLRRHD QHITAVLTKI IERDTSSVAV NLPVPSGVAF PAVFLRSRDT QGKSATSGKR SIFAQEIAAR RIAEAKGPSV GEVVPNVGPP EGAVTCETPT PRNQGCQLPG SSHSFQGPNL VTGKGLRDQE AEQEAQTIHE ENIARLQAMA PEEILQEQQR LLAQLDPSLV AFLRSHSHTQ EQTGETASEE QRPGGPSANV TKEEPLMSAF ASEPRKRDKL EPEAPALALP VTPQKEWLHM DTVELEKLHW TQDLPPVRRQ QT (1-351 aa encoded by BC000246)

Target Information

RNA polymerases transcribe nuclear genes for ribosomal RNA and are integral components of ribosomal biogenesis. RNA polymerase I (Pol I) is located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. RNA polymerase II (Pol II) synthesizes mRNA. RNA polymerase III (Pol III) transcribes class III genes, encoding a number of small ribosomal RNA molecules including tRNA and 5S rRNA. RPAP1 (RNA polymerase II associated protein 1) is a 1, 393 amino acid nuclear protein that is thought to connect Pol II to acetylated Histone H3 and regulators of protein complex formation. A member of the RPAP1 family, RPAP1 exists as three alternatively spliced isoforms that are encoded by a gene that maps to human chromosome 15q15.1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity.

Cell reports

Lynch CJ,Bernad R,Calvo I,Nóbrega-Pereira S,Ruiz S,Ibarz N,Martinez-Val A,Graña-Castro O,Gómez-López G,Andrés-León E,Espinosa Angarica V,Del Sol A,Ortega S,Fernandez-Capetillo O,Rojo E,Munoz J,Serrano M

Tue Jan 09 00:00:00 EST 2018

Bioinformatics

Protein Aliases: RNA polymerase II-associated protein 1; RPAP-1

Gene Aliases: 1190005L06Rik; A730023M06Rik; AU041788; AW107702; KIAA1403; mKIAA1403; RPAP1

UniProt ID: (Human) Q9BWH6, (Mouse) Q80TE0, (Rat) Q3T1I9

Entrez Gene ID: (Human) 26015, (Mouse) 68925, (Rat) 311338

Function(s)

transcription, DNA-templated transcription from RNA polymerase II promoter

Process(es)

DNA binding DNA-directed RNA polymerase activity protein binding transferase activity nucleotidyltransferase activity

RPAP1 Polyclonal Antibody

Product Details

15138-1-AP

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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