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Agrisera
RPB2/DNA-directed RNA polymerase II subunit 2 Polyclonal Antibody
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FIGURE: 1 / 1
RPB2/DNA-directed RNA polymerase II subunit 2 Antibody (AS20-4376) in WB
10-40 µg/well of total protein extracted from 7-d-old Arabidopsis thaliana seedlings (grown in continuous light at 22ºC) with 50 m M Tris-HCl p H 7.5,150 m M Na Cl, 0.25% Triton X-100,2 m M PMSF and 1 x protease inhibitor cocktail (Roche) and denatured with 1 XLaemLi buffer at 100°C for 5 min, were separated in duplicate on 7% SDS-PAGEs and blotted 1 h 15 min to PVDF (0.2 um), using wet transfer. Blots were... View More
Product Details
AS20-4376
Product Specifications
Species Reactivity
Plant
Host/Isotype
Chicken
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana RPB2.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hRPB140; hs hRPB140; hsRPB2; POL2RB; rpb 2; RPB2
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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