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Bethyl Laboratories
RPC3 Polyclonal Antibody
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FIGURE: 1 / 1
RPC3 Antibody (A303-063A) in IP
Detection of human RPC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RPC3 antibody (Product # A303-063A) used for IP at 6 µg/mg lysate. RPC3 was also immunoprecipitated by rabbit anti-RPC3 antibody A303-064A, which recognizes a downstream epitope. For blotting immunoprecipitated RPC3, A303-064A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A303-063A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 200 and 250 of Human Polymerase (RNA) III (DNA directed) Polypeptide C (62kD).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3C is a specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. POLR3C may direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. POLR3C may be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. POLR3C plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses.POLR3C acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3C can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-directed III 62 kDa polypeptide; DNA-directed RNA polymerase III subunit C; DNA-directed RNA polymerase III subunit RPC3; polymerase (RNA) III (DNA directed) polypeptide C (62kD); polymerase (RNA) III subunit C; RNA polymerase III 62 kDa subunit; RNA polymerase III subunit C3; RPC62
Gene Aliases: POLR3C; RPC3; RPC62
UniProt ID: (Human) Q9BUI4
Entrez Gene ID: (Human) 10623
regulation of transcription from RNA polymerase III promoter
transcription from RNA polymerase III promoter
positive regulation of type I interferon production
positive regulation of interferon-beta production
innate immune response
positive regulation of innate immune response
defense response to virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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