Proteintech

RPE65 Polyclonal Antibody

RPE65 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RPE65 Antibody (17939-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using (Product # 17939-1-AP) (RPE65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

17939-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RPE65 Fusion Protein Ag12356 (204-533 aa encoded by BC075036)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: YNIVKIPPL QADKEDPISK SEIVVQFPCS DRFKPSYVHS FGLTPNYIVF VETPVKINLF KFLSSWSLWG ANYMDCFESN ETMGVWLHIA DKKRKKYLNN KYRTSPFNLF HHINTYEDNG FLIVDLCCWK GFEFVYNYLY LANLRENWEE VKKNARKAPQ PEVRRYVLPL NIDKADTGKN LVTLPNTTAT AILCSDETIW LEPEVLFSGP RQAFEFPQIN YQKYCGKPYT YAYGLGLNHF VPDRLCKLNV KTKETWVWQE PDSYPSEPIF VSHPDALEED DGVVLSVVVS PGAGQKPAYL LILNAKDLSE VARAEVEINI PVTFHGLFKK S (204-533 aa encoded by BC075036)

Target Information

RPE65 is a major protein of the retinal pigment epithelium (RPE). RPE65 is essential for the regeneration of rhodopsin in the visual cycle. Mutations in RPE65 are responsible for certain forms of autosomal recessive severe retinal dystrophy, including Leber Congenital Amaurosis (LCA). Loss of function results in a block in retinoid processing required for the synthesis of 11-cis retinal and the accumulation of retinyl esters in the RPE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Region-resolved multi-omics of the mouse eye.

Cell reports

Xiang H,Zhang B,Wang Y,Xu N,Zhang F,Luo R,Ji M,Ding C

Tue Feb 28 00:00:00 UTC 2023

CRISPR-cas9 screening identified lethal genes enriched in Hippo kinase pathway and of predictive significance in primary low-grade glioma.

Molecular medicine (Cambridge, Mass.)

Mijiti M,Maimaiti A,Chen X,Tuersun M,Dilixiati M,Dilixiati Y,Zhu G,Wu H,Li Y,Turhon M,Abulaiti A,Maimaitiaili N,Yiming N,Kasimu M,Wang Y

Sun May 14 00:00:00 UTC 2023

Thioredoxin upregulation delays diabetes-induced photoreceptor cell degeneration via AMPK-mediated autophagy and exosome secretion.

Diabetes research and clinical practice

Ren X,Lv J,Wang N,Liu J,Gao C,Wu X,Yu Y,Teng Q,Dong W,Kong H,Kong L

Tue Mar 01 00:00:00 UTC 2022

Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.

Redox biology

Liu C,Sun W,Zhu T,Shi S,Zhang J,Wang J,Gao F,Ou Q,Jin C,Li J,Xu JY,Zhang J,Tian H,Xu GT,Lu L

Wed Jun 01 00:00:00 UTC 2022

Prph2 disease mutations lead to structural and functional defects in the RPE.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Tebbe L,Sakthivel H,Makia MS,Kakakhel M,Conley SM,Al-Ubaidi MR,Naash MI

Sun May 01 00:00:00 UTC 2022

Lutein and zeaxanthin reduce A2E and iso-A2E levels and improve visual performance in Abca4^-/-/Bco2^-/- double knockout mice.

Experimental eye research

Arunkumar R,Gorusupudi A,Li B,Blount JD,Nwagbo U,Kim HJ,Sparrow JR,Bernstein PS

Sun Aug 01 00:00:00 UTC 2021

The Construction of Retinal Pigment Epithelium Sheets with Enhanced Characteristics and Cilium Assembly Using iPS Conditioned Medium and Small Incision Lenticule Extraction Derived Lenticules.

Acta biomaterialia

Gu J,Wang Y,Cui Z,Li H,Li S,Yang X,Yan X,Ding C,Tang S,Chen J

Mon Jul 01 00:00:00 UTC 2019

Retinal Pigment Epithelium Cell Death Is Associated With NLRP3 Inflammasome Activation by All-trans Retinal.

Investigative ophthalmology & visual science

Liao Y,Zhang H,He D,Wang Y,Cai B,Chen J,Ma J,Liu Z,Wu Y

Mon Jul 01 00:00:00 UTC 2019

Conversion of all-trans-retinal into all-trans-retinal dimer reflects an alternative metabolic/antidotal pathway of all-trans-retinal in the retina.

The Journal of biological chemistry

Gao Z,Liao Y,Chen C,Liao C,He D,Chen J,Ma J,Liu Z,Wu Y

Fri Sep 14 00:00:00 UTC 2018

Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.

The American journal of pathology

Malechka VV,Moiseyev G,Takahashi Y,Shin Y,Ma JX

Sun Oct 01 00:00:00 UTC 2017

Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.

The American journal of pathology

Malechka VV,Moiseyev G,Takahashi Y,Shin Y,Ma JX

Sun Oct 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: All-trans-retinyl-palmitate hydrolase; BCO family, member 3; Lutein isomerase; Meso-zeaxanthin isomerase; modifier of retinal degeneration 1; p63; RBP-binding membrane protein; retinal pigment epithelium 65-like; retinal pigment epithelium specific protein 65; retinal pigment epithelium, 65 kDa; Retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific protein 65kDa; Retinitis pigmentos; retinitis pigmentosa 20 (autosomal recessive); Retinoid isomerohydrolase; Retinol isomerase

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Gene Aliases: 65kDa; A930029L06Rik; BCO3; LCA2; Mord1; mRPE65; rd12; RP20; RPE65; sRPE65

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UniProt ID: (Human) Q16518, (Mouse) Q91ZQ5, (Rat) O70276

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Entrez Gene ID: (Human) 6121, (Mouse) 19892, (Rat) 89826

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Function(s)

phosphatidylserine binding retinal isomerase activity oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen phosphatidylcholine binding metal ion binding all-trans-retinyl-palmitate hydrolase, 11-cis retinol forming activity all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity cardiolipin binding hydrolase activity isomerase activity oxygenase

Process(es)

retinoid metabolic process retina homeostasis neural retina development vitamin A metabolic process regulation of rhodopsin gene expression visual perception circadian rhythm insulin receptor signaling pathway retinol metabolic process retinal metabolic process detection of light stimulus involved in visual perception oxidation-reduction process retina morphogenesis in camera-type eye cellular response to electrical stimulus response to stimulus retina development in camera-type eye response to light stimulus camera-type eye development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RPE65 Polyclonal Antibody

RPE65 Antibody (17939-1-AP) in IHC (P)

Product Details

17939-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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