Invitrogen

RPE65 Polyclonal Antibody

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RPE65 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — RPE65 Polyclonal Antibody detects RPE65 protein at retinal pigment epithelium by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: RPE65 stained by RPE65 Polyclonal Antibody (Product # PA5-78414) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuronal marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

RPE65 Antibody in Western Blot (WB) — Western Blot using RPE65 Polyclonal Antibody (Product # PA5-78414). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RPE65 Polyclonal Antibody (Product # PA5-78414) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

PA5-78414

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the center region of human RPE65. The exact sequence is proprietary.
Conjugate	Unconjugated
Form	Liquid
Concentration	1.36 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2736536

Product Specific Information

Positive Control: mouse eye

Predicted Reactivity: Rat (93%), Xenopus laevis (83%), Dog (97%), Chicken (89%), Bovine (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RPE65 is a major protein of the retinal pigment epithelium (RPE). RPE65 is essential for the regeneration of rhodopsin in the visual cycle. Mutations in RPE65 are responsible for certain forms of autosomal recessive severe retinal dystrophy, including Leber Congenital Amaurosis (LCA). Loss of function results in a block in retinoid processing required for the synthesis of 11-cis retinal and the accumulation of retinyl esters in the RPE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evaluation for Retinal Therapy for RPE65 Variation Assessed in hiPSC Retinal Pigment Epithelial Cells.

Stem cells international

Nash BM,Loi TH,Fernando M,Sabri A,Robinson J,Cheng A,Eamegdool SS,Farnsworth E,Bennetts B,Grigg JR,Chung SK,Gonzalez-Cordero A,Jamieson RV

Published figure using RPE65 polyclonal antibody (Product # PA5-78414) in Western Blot

Fri Dec 24 00:00:00 EST 2021

Bioinformatics

Protein Aliases: All-trans-retinyl-palmitate hydrolase; BCO family, member 3; Lutein isomerase; Meso-zeaxanthin isomerase; modifier of retinal degeneration 1; p63; RBP-binding membrane protein; retinal pigment epithelium specific protein 65; retinal pigment epithelium, 65 kDa; Retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific protein 65kDa; Retinitis pigmentos; retinitis pigmentosa 20 (autosomal recessive); Retinoid isomerohydrolase; Retinol isomerase

Gene Aliases: 65kDa; A930029L06Rik; BCO3; LCA2; Mord1; mRPE65; rd12; RP20; RPE65; sRPE65

UniProt ID: (Human) Q16518, (Mouse) Q91ZQ5

Entrez Gene ID: (Human) 6121, (Mouse) 19892

Molecular Function: oxygenase