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Invitrogen
RPGR Polyclonal Antibody
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FIGURE: 1 / 1
RPGR Antibody (PA5-79934) in WB
Western blot analysis of RPGR in Lane 1: rat liver tissue lysate, Lane 2: rat kidney tissue lysate, Lane 3: rat brain tissue lysate, Lane 4: rat heart tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with RPGR polyclonal antibody (Product # PA5-79934) ... View More
Product Details
PA5-79934
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human RPGR recombinant protein (Position: A13-F338).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue, rat kidney tissue, rat brain tissue, rat heart tissue.
Target Information
This gene encodes a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: retinitis pigmentosa 15; retinitis pigmentosa 3 GTPase regulator; X-linked retinitis pigmentosa GTPase regulator
Gene Aliases: COD1; CORDX1; CRD; orf15; PCDX; RP15; RP3; RPGR; XLRP3
UniProt ID: (Human) Q92834
Entrez Gene ID: (Human) 6103, (Rat) 367733
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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