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Invitrogen
RPH3A Polyclonal Antibody
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FIGURE: 1 / 5
RPH3A Antibody (PA1-774) in ICC/IF
Immunofluorescence analysis of Rabphilin 3A was performed using 70% confluent log phase RSC96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RPH3A Rabbit Polyclonal Antibody (Product # PA1-774) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More
Product Details
PA1-774
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1) T D T V V N R W M Y P G D G P L Q(18) C of rat Rabphilin 3A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-774 detects rabphilin 3A from rat tissues.
PA1-774 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~75 kDa protein representing rabphilin 3A from rat brain whole protein extract.
PA1-774 immunogen is a synthetic peptide corresponding to residues M(1) T D T V V N R W M Y P G D G P L Q(18) C of rat Rabphilin 3A. This sequence is completely conserved between rat and mouse rabphilin 3A. PA1-774 immunizing peptide (Cat. # PEP-102) is available for use in neutralization and control experiments.
Target Information
The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B, and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Exophilin 1; Exophilin-1; Exophilin1; rabphilin 3A homolog; Rabphilin-3A
Gene Aliases: 2900002P20Rik; AU022689; AW108370; Rph3a
UniProt ID: (Rat) P47709, (Mouse) P47708
Entrez Gene ID: (Rat) 171039, (Mouse) 19894
transporter activity
calcium ion binding
protein binding
phospholipid binding
calcium-dependent phospholipid binding
phosphatidylinositol-4,5-bisphosphate binding
zinc ion binding
selenium binding
Rab GTPase binding
protein complex binding
phosphate ion binding
metal ion binding
inositol 1,4,5 trisphosphate binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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