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Invitrogen
RPH3A Polyclonal Antibody
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FIGURE: 1 / 4
RPH3A Antibody (PA5-35960) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of RPH3A was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RPH3A Polyclonal Antibody (Product # PA5-35960) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-35960
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Rabphilin 3A. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
Product Specific Information
Recommended positive controls: mouse brain, rat brain.
Predicted reactivity: Mouse (88%), Rat (88%), Bovine (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B, and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Exophilin 1; Exophilin-1; Exophilin1; rabphilin 3A homolog; Rabphilin-3A; unnamed protein product
Gene Aliases: 2900002P20Rik; AU022689; AW108370; KIAA0985; RPH3A
UniProt ID: (Human) Q9Y2J0, (Rat) P47709, (Mouse) P47708
Entrez Gene ID: (Human) 22895, (Rat) 171039, (Mouse) 19894
calcium ion binding
protein binding
calcium-dependent phospholipid binding
phosphatidylinositol-4,5-bisphosphate binding
zinc ion binding
lipid binding
selenium binding
small GTPase binding
phosphate ion binding
macromolecular complex binding
metal ion binding
inositol 1,4,5 trisphosphate binding
phosphatidylinositol phosphate binding
transporter activity
phospholipid binding
Rab GTPase binding
protein complex binding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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