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          • Primary Antibodies ›
          • RPL10 Antibodies

          Invitrogen

          RPL10 Polyclonal Antibody

          View all (12) RPL10 antibodies

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          Cite RPL10 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (7)
          RPL10 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          RPL10 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          RPL10 Antibody (PA5-143918) in ICC/IF

          Immunocytochemistry analysis of RPL10 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL RPL10 polyclonal antibody (Product # PA5-143918) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the la... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          RPL10 Antibody in Immunocytochemistry (ICC/IF)
          RPL10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RPL10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RPL10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RPL10 Antibody in Western Blot (WB)
          RPL10 Antibody in Flow Cytometry (Flow)
          RPL10 Antibody in Flow Cytometry (Flow)
          RPL10 Polyclonal Antibody

          Product Details

          PA5-143918

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-0.5 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          2-5 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human RPL10 recombinant protein (Position: R3-S214).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3075132

          Product Specific Information

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human Hela whole cell, human K562 whole cell, human HEK293 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell. IHC: human gall bladder adenosquamous carcinoma tissue, mouse brain tissue, rat brain tissue. ICC/IF: MCF-7 cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          This gene encodes a ribosomal protein that is a component of the 60S ribosome subunit. The related protein in chicken can bind to c-Jun and can repress c-Jun-mediated transcriptional activation. Some studies have detected an association between variation in this gene and autism spectrum disorders, though others do not detect this relationship. There are multiple pseudogenes of this gene dispersed throughout the genome. Alternative splicing results in multiple transcript variants.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 24.6 kDa protein; 24.6kDa protein; 60S ribosomal protein L10; Laminin receptor homolog; laminin receptor homolog clone A; Large ribosomal subunit protein uL16; may code for Wilm's tumor-related protein; Protein QM; Protein QM homolog; ribosomal protein 10; Ribosomal protein L10; RPL10; RPLPO; Tumor suppressor QM; unnamed protein product; Wilms tumor-related protein; Wilms' tumor suppressor

          View more View less

          Gene Aliases: AUTSX5; D0HXS648; DXHXS648; DXHXS648E; DXS648; DXS648E; L10; MRXS35; NOV; QM; QM16; RPL10; uL16

          View more View less

          UniProt ID: (Human) P27635, (Rat) Q6PDV7, (Mouse) Q6ZWV3

          View more View less

          Entrez Gene ID: (Human) 6134, (Rat) 81764, (Mouse) 110954

          View more View less

          Function(s)
          RNA binding structural constituent of ribosome protein binding translation regulator activity poly(A) RNA binding ribosomal protein
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter cytoplasmic translation translation regulation of translation negative regulation of apoptotic process embryonic brain development
          It has to be done as per old AB suggested Products section.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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